首页> 外文期刊>Food microbiology >Comparison of molecular detection methods for Vibrio parahaemolyticus and Vibrio vulnificus
【24h】

Comparison of molecular detection methods for Vibrio parahaemolyticus and Vibrio vulnificus

机译:副溶血性弧菌和创伤弧菌的分子检测方法比较

获取原文
获取原文并翻译 | 示例
           

摘要

Pathogenic vibrios are a global concern for seafood safety and many molecular methods have been developed for their detection. This study compares several molecular methods for detection of total and pathogenic Vibrio parahaemolyticus and Vibrio vulnificus, in MPN enrichments from oysters and fish intestine samples. This study employed the DuPont Qualicon BAX~R System Real-Time PCR assay for detection of V. parahaemolyticus and V. vulnificus. Multiplex real-time PCR detection of total (tlh+), tdh+, and trh+ V. parahaemolyticus was conducted on the Cepheid SmartCycler II. Total (rpoD) and tdh+ V. parahaemolyticus were also detected using LAMP. V. vulnificus detection was performed using real-time PCR methods developed for the SmartCycler and the AB 7500 Fast. Recommended template preparations were compared to BAX~R lysis samples for suitability. There was no significant difference in detection of V. parahaemolyticus and V. vulnificus using the BAX~R or SmartCycler assays. The AB assay showed no difference from other methods in detection of V. vulnificus unless boiled templates were utilized. There was a significant difference in detection of tdh+ V. parahaemolyticus between SmartCycler and LAMP assays unless the total (tlh+) V. parahaemolyticus gene target was omitted from the SmartCycler assay; a similar trend was observed for trh+ V. parahaemolyticus.
机译:致病性弧菌是海产品安全性的全球关注点,并且已经开发出许多检测它们的分子方法。这项研究比较了从牡蛎和鱼肠样品中MPN富集的总分子和致病性副溶血弧菌和创伤弧菌的几种分子检测方法。本研究采用杜邦Qualicon BAX〜R系统实时荧光定量PCR检测副溶血性弧菌和创伤性弧菌。在Cepheid SmartCycler II上对总(tlh +),tdh +和trh +副溶血性弧菌进行了多重实时PCR检测。还使用LAMP检测了总(rpoD)和tdh +副溶血性弧菌。使用为SmartCycler和AB 7500 Fast开发的实时PCR方法进行创伤弧菌检测。将推荐的模板制备物与BAX〜R裂解样品进行比较以确保适用性。使用BAX〜R或SmartCycler分析检测副溶血性弧菌和创伤性弧菌没有显着差异。除非使用煮沸的模板,否则AB分析显示在检测V. vulnificus方面与其他方法没有差异。除非SmartCycler分析中省略了总(tlh +)副溶血弧菌基因靶点,否则SmartCycler和LAMP分析之间tdh +副溶血弧菌的检测存在显着差异。对于trh +副溶血性弧菌,也观察到了类似的趋势。

著录项

  • 来源
    《Food microbiology》 |2012年第1期|p.105-111|共7页
  • 作者单位

    FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, I lberville Drive, Dauphin Island, AL 36528, USA;

    National Institute of Health Sciences, Division of Microbiology, Setagayaku, Tokyo 158 8501, Japan;

    FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, I lberville Drive, Dauphin Island, AL 36528, USA;

    FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, I lberville Drive, Dauphin Island, AL 36528, USA;

    DuPont Qualicon, Rt. 41 & Henry Clay Road, Wilmington, DE 19880, USA;

    DuPont Qualicon, Rt. 41 & Henry Clay Road, Wilmington, DE 19880, USA;

    FDA, CFSAN, 5100 Paint Branch Parkway, College Park, MD 20740, USA;

    FDA, Division of Seafood Science and Technology, Gulf Coast Seafood Laboratory, I lberville Drive, Dauphin Island, AL 36528, USA;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    vibrio vulnificus; vibrio parahaemolyticus; real-time PCR; loop-mediated isothermal amplification; (LAMP);

    机译:创伤弧菌;副溶血性弧菌实时PCR;环介导的等温扩增;(灯);

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号