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首页> 外文期刊>Food microbiology >Contribution of the NADH-oxidase (Nox) to the aerobic life of Lactobadllus sanfranciscensis DSM20451~T
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Contribution of the NADH-oxidase (Nox) to the aerobic life of Lactobadllus sanfranciscensis DSM20451~T

机译：NADH-氧化酶（Nox）对旧金山乳杆菌DSM20451〜T好氧生活的贡献

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摘要

Lactobadllus sanfranciscensis is the key bacterium in traditional sourdough fermentation. The molecular background of its oxygen tolerance was investigated by comparison of wild type and NADH-oxidase (Nox) knock out mutants. The nox gene of L. sanfranciscensis DSM20451~T coding for a NADH-oxidase (Nox) was inactivated by single crossover integration to yield strain L sanfranciscensis DSM20451Anox. By inactivation of the native NADH-oxidase gene, it was ensured that besides fructose, O_2 can react as an electron acceptor. In aerated cultures the mutant strain was only able to grow in MRS media supplemented with fructose as electron acceptor, whereas the wild type strain showed a fructose independent growth response. The use of oxygen as an external electron acceptor enables L sanfranciscensis to shift from acetyl-phosphate into the acetate branch and gain an additionally ATP, while the reduced cofactors were regenerated by Nox-activity. In aerated cultures the wild type strain formed a fermentation ratio of lactate to acetate of 1.09 in MRS supplemented with fructose after 24 h of fermentation, while the mutant strain formed a fermentation ratio of 3.05. Additionally, L sanfranciscensis showed manganese-dependent growth response in aerated cultures, the final OD and growth velocity was increased in media supplemented with manganese. The expression of two predicted Mn~(2+)/Fe~(2+) transporters MntHl and MntH2 in L. sanfranciscensis DSM20451~T was verified by amplification of a 318 bp fragment of MntHl and a 239 bp fragment of MntH2 from cDNA library obtained from aerobically, exponentially growing cells of L sanfranciscensis DSM20451~T in MRS. Moreover, the mutant strain DSM20451Anox was more sensitive to the superoxide generating agent paraquat and showed inhibition of growth on diamide-treated MRS-plates without fructose supplementation.
机译：Sanfranciscensis乳杆菌是传统酵母发酵中的关键细菌。通过比较野生型和NADH-氧化酶（Nox）敲除突变体调查了其耐氧性的分子背景。通过单交换整合失活编码L. Sanfranciscensis DSM20451〜T的nox基因的NADH氧化酶（Nox），以产生菌株L Sanfranciscensis DSM20451Anox。通过使天然的NADH-氧化酶基因失活，确保除了果糖以外，O_2还可以作为电子受体反应。在充气培养中，突变菌株只能在补充有果糖作为电子受体的MRS培养基中生长，而野生型菌株显示出果糖非依赖性的生长反应。使用氧作为外部电子受体可使三叶兰花从乙酰磷酸盐转变为乙酸盐分支并获得额外的ATP，而还原的辅因子则通过Nox活性再生。在充气培养中，野生型菌株在发酵24小时后在补充果糖的MRS中形成的乳酸与乙酸的发酵比为1.09，而突变菌株形成的发酵比为3.05。此外，L.franciscensis在充气培养物中显示出锰依赖性的生长反应，在补充锰的培养基中最终的OD和生长速度增加。通过扩增cDNA文库中的MntH1的318 bp片段和MntH2的239 bp片段，验证了三株L. sanfranciscensis DSM20451〜T中预测的Mn〜（2 +）/ Fe〜（2+）转运蛋白MntH1和MntH2的表达。得自MRS中Sanfranciscensis DSM20451〜T的需氧，指数生长的细胞。此外，突变菌株DSM20451Anox对超氧化物生成剂百草枯更敏感，并且在未添加果糖的情况下显示了在经二酰胺处理的MRS平板上的生长抑制。

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  • 来源
    《Food microbiology》 |2011年第1期|p.29-37|共9页
  • 作者

    Andre Jaensch; Simone Freiding; Juergen Behr; Rudi F. Vogel;

  • 作者单位

    Technische Universitat Miinchen, Lehrstuht Technische Mikrobiologie, Weihenstephaner Steig 16, D-85350 Freising. Germany;

    rnTechnische Universitat Miinchen, Lehrstuht Technische Mikrobiologie, Weihenstephaner Steig 16, D-85350 Freising. Germany;

    rnTechnische Universitat Miinchen, Lehrstuht Technische Mikrobiologie, Weihenstephaner Steig 16, D-85350 Freising. Germany;

    rnTechnische Universitat Miinchen, Lehrstuht Technische Mikrobiologie, Weihenstephaner Steig 16, D-85350 Freising. Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    lactobadllus sanfranciscensis; NADH-oxidase; manganese; oxygen tolerance;

    机译：旧金山乳杆菌;NADH-氧化酶;锰;耐氧性;

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