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首页> 外文期刊>Food microbiology >Comparison Of Culture Media For Enrichment And Isolation Of Salmonella Spp. From Frozen Channel Catfish And Vietnamese Basa Fillets
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Comparison Of Culture Media For Enrichment And Isolation Of Salmonella Spp. From Frozen Channel Catfish And Vietnamese Basa Fillets

机译：沙门氏菌富集和分离培养基的比较。从冷冻Channel鱼和越南巴沙鱼片

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摘要

Frozen fillets of Channel catfish and Vietnamese basa-fish were used to compare Salmonella spp. recovery effectiveness of selective enrichment in Rappaport-Vassiliadis (RV) broth and tetrathionate broth (TT) and selective isolation on Hekteon enteric (HE) agar, xylose lysine deoxycholate (XLD) agar, and bismuth sulfite (BS) agar. Isolate confirmation was through fatty acid methyl ester analysis. Of 60 samples analyzed, 25 were found contaminated with Salmonella (42% incidence). Salmonella spp. recovery after enrichment in RV medium was 35% on HE agar, 30% on XLD agar, and 42% on BS agar. Similarly, after enrichment in TT broth, HE and XLD agars recovered 22% each and BS agar recovered 37%. No performance difference (p > 0.05) was observed in the recovery of Salmonella using the combinations of BS, HE, and XLD agars with RV broth and BS agar with TT broth. The combination of selective enrichment in RV and selective isolation on BS gave numerically greatest isolation of Salmonella from Channel catfish and Vietnamese basa fish compared to other isolation combinations.
机译：用海峡cat鱼和越南ba鱼的冷冻鱼片比较沙门氏菌。 Rappaport-Vassiliadis（RV）肉汤和四硫酸盐肉汤（TT）中选择性富集的回收效果，以及对Hekteon肠溶（HE）琼脂，木糖赖氨酸脱氧胆酸盐（XLD）琼脂和亚硫酸铋（BS）琼脂的选择性分离。分离物确认是通过脂肪酸甲酯分析。在分析的60个样本中，有25个被沙门氏菌污染（发生率42％）。沙门氏菌在RV培养基中富集后，HE琼脂的回收率为35％，XLD琼脂的回收率为30％，BS琼脂的回收率为42％。同样，富集TT肉汤后，HE和XLD琼脂分别回收22％，BS琼脂回收37％。结合使用BS，HE和XLD琼脂与RV肉汤以及BS琼脂与TT肉汤的组合，沙门氏菌的回收率未观察到性能差异（p> 0.05）。与其他隔离组合相比，RV选择性富集和BS选择性隔离的组合在数值上最大程度地从海cat鱼和越南Vietnamese鱼中分离出沙门氏菌。

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