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Culture-independent methods for identifying microbial communities in cheese

机译:与文化无关的方法,用于鉴定奶酪中的微生物群落

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This review focuses on the culture-independent methods available for the description of both bacterial and fungal communities in cheese. Important steps of the culture-independent strategy, which relies on bulk DNA extraction from cheese and polymerase chain reaction (PCR) amplification of selected sequences, are discussed. We critically evaluate the identification techniques already used for monitoring microbial communities in cheese, including PCR-denaturing gradient gel electrophoresis (PCR-DGGE), PCR-temporal temperature gradient gel electrophoresis (PCR-TTGE) or single-strand conformation polymorphism-PCR (SSCP-PCR) as well as some other techniques that remain to be adapted to the study of cheese communities. Further, our analysis draws attention to the lack of data available on suitable DNA sequences for identifying fungal communities in cheese and proposes some potential DNA targets.
机译:这篇综述集中在可用于描述奶酪中细菌和真菌群落的文化独立方法上。讨论了不依赖于培养物的策略的重要步骤,该步骤依赖于从干酪中大量提取DNA和所选序列的聚合酶链反应(PCR)扩增。我们严格评估已经用于监测奶酪中微生物群落的鉴定技术,包括PCR变性梯度凝胶电泳(PCR-DGGE),PCR时间温度梯度凝胶电泳(PCR-TTGE)或单链构象多态性PCR(SSCP) -PCR)以及其他一些仍需适应奶酪研究的技术。此外,我们的分析引起人们对缺乏合适的DNA序列数据来识别奶酪中真菌群落的关注,并提出了一些潜在的DNA靶标。

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