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RAPD with micro satellite as a tool for differentiation of Candida genus yeasts isolated in brewing

机译：带有微型卫星的RAPD作为区分酿造过程中念珠菌属酵母的工具

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摘要

Fifteen wild yeast strains were isolated in two factories of a lager brewing company in Poland. Their identification with API 32C system showed mainly the presence of Candida sake species (7/15). To differentiate the isolates, randomly amplified polymorphic DNA (RAPD) with (GTG)_5, (GAC)_5, (GACA)_4 microsatellite primers and M13 core sequence (5'-GAG GGT GGC GGT TCT-3') were chosen. The results of patterns similarity are presented as dendrograms for each RAPD analysis and for overall patterns. On the overall patterns, all isolates identified as C. sake, except Strain No. 1, were regrouped in one cluster. Collection strain C. sake CBS 617 was similar in 46% to the cluster with six isolates (Strain Nos. 3, 6, 8, 11, 13, 14). The second reference strain C. sake CBS 159 and the Strain No. 1 were regrouped with other Candida species (collection strains) showing, respectively, only 20% and 42% of similarity to other C. sake strains. The similarity based on the overall dendrogram between isolate Nos. 3, 6, 8, 11, 13, 14 and C. sake CBS 617 was 49%. Between those strains and other Candida, the similarity was only 37%.
机译：在波兰一家大型啤酒酿造公司的两家工厂中分离出了15种野生酵母菌株。他们用API 32C系统鉴定主要显示了假丝酵母菌的存在（7/15）。为了区分分离物，选择了具有（GTG）_5，（GAC）_5，（GACA）_4微卫星引物和M13核心序列（5'-GAG GGT GGC GGT TCT-3'）的随机扩增多态性DNA（RAPD）。模式相似性的结果显示为每个RAPD分析和整体模式的树状图。在总体模式上，除1号菌株外，所有鉴定为酿酒酵母的分离株都重新分组为一个簇。收集菌株清酒梭菌CBS 617与含有六个分离株的菌株（菌株3、6、8、11、13、14）的相似性为46％。第二参考菌株清酒丁香梭菌CBS 159和1号菌株与其他假丝酵母菌种（收集菌株）重新组合，分别显示出与其他清酒丁香菌株的相似性仅为20％和42％。基于3号，6号，8号，11号，13号，14号分离物与清酒假丝酵母CBS 617之间总体树状图的相似度为49％。这些菌株与其他念珠菌之间的相似度仅为37％。

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