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首页> 外文期刊>Food microbiology >Prevalence and level of Escherichia coli O157 on beef trimmings, carcasses and boned head meat at a beef slaughter plant.
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Prevalence and level of Escherichia coli O157 on beef trimmings, carcasses and boned head meat at a beef slaughter plant.

机译：牛肉屠宰场中的牛肉装饰物，尸体和去骨头肉中大肠杆菌O157的患病率和水平。

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This study investigated the prevalence and level of Escherichia coli O157 on samples of beef trimmings (n=1351), beef carcasses (n=132) and bovine head meat (n=132) in a beef slaughter plant in Ireland. The survey also included an assessment of the prevalence of virulence genes in the E. coli O157 isolates obtained. Samples were examined for the presence of E. coli O157 by direct plating on SMAC-CT and by enrichment/immunomagnetic separation (IMS) with plating of recovered immunobeads onto SMAC-CT agar. Presumptive E. coli O157 isolates were confirmed by PCR targeting a range of genes i.e. vt1, vt2, eaeA, hlyA, fliCh7 and portions of the rfb (O-antigen encoding) region of E. coli O157. Enterobacteriaceae on head meat samples were estimated by direct plating onto Violet Red Bile Glucose agar. E. coli O157 was recovered from 2.4% (32/1351) of beef trimmings samples, at concentrations ranging from <0.70-1.61 log10 cfu g-1. Of the 32 positive isolates, 31 contained the eaeA and hylA genes while 30/32 contained the fliCh7 gene and 31/32 contained vt1 or vt2, or both vt genes. E. coli O157 was recovered from 3.0% (4/132) of carcass samples, at concentrations ranging from <0.70-1.41 log10 cfu g-1. All of the carcass isolates contained the eaeA, hylA and fliCh7 genes. E. coli O157 was recovered from 3.0% (3/100) of head meat samples, at concentrations of 0.7-1.0 log10 cfu g-1. All of the head meat isolates contained the eaeA, hylA, fliCh7 and vt2 genes. No head meat isolates contained the vt1 gene. Head meat samples (n=100) contained Enterobacteriaceae, at concentrations ranging from 0.70-3.0 log10 cfu g-1. Overall, the qualitative and quantitative data obtained for E. coli O157 on beef trimming samples in this study could be employed as part of a quantitative risk assessment model..
机译：这项研究调查了爱尔兰一家牛肉屠宰厂的牛肉装饰物（n = 1351），牛肉beef体（n = 132）和牛头肉（n = 132）样本中大肠杆菌O157的流行和水平。该调查还包括评估获得的大肠杆菌O157分离物中毒力基因的流行程度。通过直接铺板在SMAC-CT上并通过富集/免疫磁分离（IMS）并将回收的免疫珠铺板到SMAC-CT琼脂上来检查样品中是否存在大肠杆菌O157。通过PCR证实推定的大肠杆菌O157分离株靶向一系列基因，即vt1，vt2，eaeA，hlyA，fliCh7和大肠杆菌O157的rfb（O-抗原编码）区域的部分。通过直接铺板在紫红色胆汁葡萄糖琼脂上估计头肉样品上的肠杆菌科。从2.4％（32/1351）的牛肉屑样品中回收了大肠杆菌O157，浓度范围为<0.70-1.61 log10 cfu g-1。在32个阳性分离株中，有31个包含eaeA和hylA基因，而30/32包含fliCh7基因，而31/32包含vt1或vt2或两个vt基因。从3.0％（4/132）的cas体样品中回收大肠杆菌O157，浓度范围为<0.70-1.41 log10 cfu g-1。所有the体分离物均包含eaeA，hylA和fliCh7基因。从3.0％（3/100）的头肉样品中回收大肠杆菌O157，浓度为0.7-1.0 log10 cfu g-1。所有头肉分离株均包含eaeA，hylA，fliCh7和vt2基因。没有头肉分离株包含vt1基因。头肉样品（n = 100）包含肠杆菌科，浓度范围为0.70-3.0 log10 cfu g-1。总的来说，这项研究中从大肠杆菌O157获得的关于牛肉修整样品的定性和定量数据可以用作定量风险评估模型的一部分。

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