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Isolation and characterization of Shiga toxin-producing Escherichia coli from precooked sausages (morcillas)

机译:从预煮香肠中提取产志贺毒素的大肠杆菌的方法

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摘要

The aim of the study was to establish the microbiological quality of morcillas, typical Argentine sausages, and to investigate the presence of Shiga toxin-producing Escherichia coli (STEC). Between October 2001 and October 2002, a total of 100 morcilla samples were analysed. Several samples showed high levels of total aerobic mesophilic bacteria counts, molds and yeasts. The samples analysed contained Enterobacteriaceae (100%) and fecal coliforms (81%), indicating inadequate application of the thermal treatment and deficient hygiene conditions during the elaboration of the product. STEC strains were isolated from three out of 100 (3%) morcilla samples. Two strains (2%) were characterized as E. coli O157:H7 stx2 + stx2vh-a/eae/EHEC-hlyA and one strain (1%), as E. coli O26:H11 stx1/eae/EHEC-hlyA. Considering both the high microbial count in all tested samples and the presence of STEC strains in three of them, morcillas should be considered a food unsafe to consume when inadequately cooked.
机译:该研究的目的是建立典型的阿根廷香肠小香肠的微生物质量,并调查产生志贺毒素的大肠杆菌(STEC)的存在。在2001年10月至2002年10月之间,总共分析了100份莫尔西拉样品。几个样品显示出高水平的总需氧中温细菌数,霉菌和酵母菌。分析的样品中含有肠杆菌科细菌(100%)和粪大肠菌(81%),表明在产品加工过程中热处理应用不充分,卫生条件不足。从100份(3%)的桑树样本中分离出STEC菌株。两种菌株(2%)的特征为大肠杆菌O157:H7 stx2 + stx2vh-a / eae / EHEC-hlyA,一种菌株(1%)的特征为大肠杆菌O26:H11 stx1 / eae / EHEC-hlyA。考虑到所有测试样品中的微生物数量都很高,并且其中三个样品中都存在STEC菌株,因此,如果烹饪不充分,则应将小食视为食用不安全的食品。

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