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首页> 外文期刊>Food microbiology >Influence of acidulant identity on the effects of pH and acid resistance on the radiation resistance of Escherichia coli O157:H7
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Influence of acidulant identity on the effects of pH and acid resistance on the radiation resistance of Escherichia coli O157:H7

机译：酸化剂身份对pH值和耐酸性对大肠杆菌O157：H7辐射抗性的影响

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摘要

The effects of pH (4.0-5.5), acid identity (acetic, citric, lactic, malic, and hydrochloric), and the induction of pH-dependent stationary phase acid resistance on the radiation resistance of E. coli O157:H7 Ent-C9490 was studied using cells grown in Tryptic Soy Broth with and without dextrose (induced and non-induced to acid resistance) and then resuspended in brain heart infusion broth containing 5 g/l of an organic acid and acidified with concentrated hydrochloric acid. After treatment with gamma radiation, the number of survivors was determined by plating on brain-heart infusion agar (injured and non-injured cells) and MacConkey agar (non-injured cells), and the data used to calculate radiation D-values. The induction of pH-dependent stationary phase acid resistance consistently provided the enterohemorrhagic E. coli strain cross-protection from subsequent irradiation, increasing radiation D-values by 1.2-3.3-fold, depending on the organic acid present. The radiation resistance of E. coli, varied with acid identity, but was largely unaffected by pH within the range examined. The results indicate that induction of cross-protection resulting from induction of acid resistance is a factor that should be considered to accurately determine the radiation dose needed to inactivate enterohemorrhagic E. coli in foods.
机译：pH（4.0-5.5），酸身份（乙酸，柠檬酸，乳酸，苹果酸和盐酸）的影响以及pH依赖的固定相耐酸性的诱导对大肠杆菌O157：H7 Ent-C9490的抗辐射性的影响使用在胰蛋白酶大豆肉汤中有或没有葡萄糖（诱导和非诱导的耐酸性）生长的细胞进行研究，然后将其重悬于含有5 g / l有机酸并用浓盐酸酸化的脑心浸液中。用伽玛射线治疗后，通过在脑-心脏浸液琼脂（受伤和未受伤的细胞）和MacConkey琼脂（未受伤的细胞）上平板接种，确定存活人数，并将数据用于计算放射线D值。依赖于pH的固定相耐酸性的诱导始终为肠出血性大肠埃希氏菌菌株提供免受后续辐射的交叉保护，从而使辐射D值增加1.2-3.3倍，具体取决于存在的有机酸。大肠杆菌的抗辐射性随酸的种类而变化，但在所考察的范围内不受pH的影响很大。结果表明，由诱导耐酸性引起的交叉保护作用是一个因素，应准确地确定灭活食物中肠出血性大肠杆菌所需的辐射剂量。

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