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Optimization for the maximum bacteriocin production of Lactobacillus brevis DF01 using response surface methodology

机译：使用响应面法优化短乳杆菌DF01的最大细菌素产量

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摘要

To enhance the production of bacteriocin DF01, produced by Lactobacillus brevis DF01, cultivation conditions and medium composition were optimized by using response surface methodology (RSM). The selected 5 factors based on MRS medium were glucose, yeast extract, MgSO4, temperature, and initial pH. Fractional factorial design (FFD) was effective in searching for the main factors. By a 25−1 FFD, glucose, yeast extract, and initial pH were found to be significant factors and had positive effects on bacteriocin production. The effects of the 3 main factors on bacteriocin production were further investigated by a central composite design (CCD). RSM revealed that the maximum bacteriocin production was achieved at yeast extract concentration of 14.56 g/L, glucose concentration of 28.95 g/L, and initial pH of 6.8. After RSM, the titer of bacteriocin was increased by 4-fold.
机译：为了提高短乳杆菌DF01产生的细菌素DF01的产量，使用响应面方法（RSM）优化了培养条件和培养基组成。基于MRS培养基选择的5个因素是葡萄糖，酵母提取物，MgSO4 ，温度和初始pH。分数阶乘设计（FFD）可有效地搜索主要因素。通过25−1 FFD，发现葡萄糖，酵母提取物和初始pH值是重要因素，并且对细菌素产生具有积极影响。通过中央复合设计（CCD）进一步研究了3个主要因素对细菌素产生的影响。 RSM显示，在酵母提取物浓度为14.56 g / L，葡萄糖浓度为28.95 g / L，初始pH为6.8的情况下，细菌素产量最高。 RSM后，细菌素的效价提高了4倍。

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