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Purification and Identification of Novel Antioxidant Peptides from Enzymatic Hydrolysate of Ginkgo biloba Seed Proteins

机译:银杏种子蛋白酶解物中新型抗氧化肽的纯化与鉴定

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摘要

Ginkgo biloba seed proteins were hydrolyzed using alkali protease and pepsin to obtain antioxidant peptides. Ginkgo biloba antioxidant peptides (GKAPs) were separated by Sephadex G-25 and Sephadex G-10 gel filtration chromatography. The GKAP B3 exhibited highest antioxidant activity. B3 peptide was separated by reversed-phase high-performance liquid chromatography (RP-HPLC), and 2 peptides with good antioxidant activity, i.e., GKAPs C8 and C9, were obtained. The molecular weight and amino acid sequences of the peptides were identified by Liquid chromatography quadrupole time-of-flight mass spec-trometry (LC-Q-TOF-MS). The results showed that the molecular mass of GKAP C8 was 452.21 Da and the amino acid sequence was YVGD (Tyr-Val-Gly-Asp), while the molecular mass of GKAP C9 was 988.49 Da and the amino acid sequence was LGNTDYAVH (Leu-Gly-Asn-Thr-Asp-Tyr-Aia-Val-His). GKAPs C8 and C9 exhibited good free radical-scavenging effect and inhibited the autoxidation of the linoleic acid system.
机译:使用碱性蛋白酶和胃蛋白酶水解银杏种子蛋白,以获得抗氧化剂肽。通过Sephadex G-25和Sephadex G-10凝胶过滤色谱分离银杏抗氧化剂肽(GKAP)。 GKAP B3表现出最高的抗氧化活性。用反相高效液相色谱(RP-HPLC)分离B3肽,得到2种具有良好抗氧化活性的肽,即GKAP C8和C9。通过液相色谱四极杆飞行时间质谱(LC-Q-TOF-MS)鉴定肽的分子量和氨基酸序列。结果显示,GKAP C8的分子量为452.21 Da,氨基酸序列为YVGD(Tyr-Val-Gly-Asp),而GKAP C9的分子量为988.49 Da,氨基酸序列为LGNTDYAVH(Leu- Gly-Asn-Thr-Asp-Tyr-Aia-Val-His)。 GKAP C8和C9表现出良好的清除自由基的作用,并抑制了亚油酸系统的自氧化。

著录项

  • 来源
    《Food science and technology research》 |2013年第6期|1029-1035|共7页
  • 作者单位

    College of forest resources and environment, Nanjing forestry university, Nanjing, 210037, China;

    College of forest resources and environment, Nanjing forestry university, Nanjing, 210037, China,Institute of Food and Nutritional Engineering, Jiangsu Food Science College, Huaian, 223003, China;

    College of forest resources and environment, Nanjing forestry university, Nanjing, 210037, China;

    College of forest resources and environment, Nanjing forestry university, Nanjing, 210037, China;

    College of forest resources and environment, Nanjing forestry university, Nanjing, 210037, China;

    College of Food and Drug, Anhui Science and Technology University, Fengyang, 233100, China;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Ginkgo biloba seed protein; antioxidant peptide; radical scavenging activity; amino acid sequence;

    机译:银杏种子蛋白;抗氧化剂肽自由基清除活性;氨基酸序列;

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