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Assessment of the genetic diversity in a collection of Erianthus arundinaceus

机译:斑纹鸢尾菌的遗传多样性评估

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摘要

Erianthus arundinaceus, a wild relative of sugarcane has a number of traits including tolerance to environmental stresses desired by sugarcane breeders. The value of genetic diversity studies in providing useful information for introgression breeding and germplasm conservation is well known. A previous study reported little genetic diversity in a number of Indonesian E. arundinaceus while Indian E . arundinaceus was more diverse. A small number of Chinese E. arundinaceus was observed as being relatively diverse. However, an assessment of the genetic diversity of E. arundinaceus from the major geographical areas in the world has not been reported before. In this study, 207 E. arundinaceus accessions from China, Indonesia, India, New Guinea, Philippines and Vietnam were sampled for genetic diversity analysis using AFLP markers. Three AFLP primer combinations generated 286 markers in total, with the percentage of polymorphism observed being 99.3%. A dendrogram and PCA diagram were constructed based on the matrix of similarity analyzed with UPGMA method by NTSYS software. The results showed that: (1) a high level of diversity found within the Chinese collection compared with the Indonesian collection and (2) there was a general division amongst clones from China based on those collected from eastern versus western parts of the country, and clones from Yunnan province (western China) were the most diverse.
机译:甘蔗的野生近缘种Erianthus arundinaceus具有许多特征,包括对甘蔗育种者所需的环境胁迫的耐受性。遗传多样性研究在为渗入育种和种质保护提供有用信息方面的价值是众所周知的。先前的一项研究报告称,印度尼西亚的许多印度尼西亚阿鲁迪纳斯E. arundinaceus的遗传多样性很少。 arundinaceus更加多样化。观察到少量的中国象鼻大肠杆菌相对多样化。但是,以前尚未报道过对世界主要地理区域的金毛狗遗传多样性的评估。在这项研究中,使用AFLP标记对来自中国,印度尼西亚,印度,新几内亚,菲律宾和越南的207株金合欢蘑菇进行了遗传多样性分析。三种AFLP引物组合总共产生286个标记,观察到的多态性百分比为99.3%。根据NTSYS软件通过UPGMA方法分析的相似矩阵,建立树状图和PCA图。结果表明:(1)与印度尼西亚的物种相比,中国的物种具有较高的多样性;(2)根据从该国东部和西部地区采集的克隆,中国的克隆之间存在大致的区分,并且来自云南省(中国西部)的克隆最多。

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  • 来源
    《Genetic Resources and Crop Evolution》 |2012年第7期|p.1483-1491|共9页
  • 作者单位

    School of Life Sciences, Yunnan University, 2 Northern Cuihu Road, Kunming, 650091, Yunnan, People’s Republic of China;

    CSIRO Division of Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, QLD, 4067, Australia;

    Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane, Yunnan Sugarcane Research Institute of Yunnan Academy of Agriculture Sciences, 363 Liquan Rd, Kaiyuan, Yunnan, 661600, People’s Republic of China;

    BSES Limited Mackay, Peak Downs Highway, Te Kowai, QLD, 4741, Australia;

    Yunnan Provincial Key Laboratory of Genetic Improvement for Sugarcane, Yunnan Sugarcane Research Institute of Yunnan Academy of Agriculture Sciences, 363 Liquan Rd, Kaiyuan, Yunnan, 661600, People’s Republic of China;

    CSIRO Division of Plant Industry, Queensland Bioscience Precinct, 306 Carmody Road, St Lucia, QLD, 4067, Australia;

    Key Lab of Southwestern Crop Gene Resourc;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    AFLPs; Erianthus arundinaceus; Genetic diversity; Sugarcane;

    机译:AFLPs;赤眼莲;遗传多样性;甘蔗;

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