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首页> 外文期刊>International Journal for Ion Mobility Spectrometry >Rapid detection of synthetic biomarkers of Escherichia coli in water using microAnalyzer: a field dependence study
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Rapid detection of synthetic biomarkers of Escherichia coli in water using microAnalyzer: a field dependence study

机译：使用microAnalyzer快速检测水中的大肠杆菌合成生物标志物：一项领域依赖性研究

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摘要

Mixtures of 12 known volatile metabolites of Escherichia coli (2,5-dimethyltetrahydrofuran, dimethyl disulfide, 2-heptanone, 2,5-dimethylpyrazine, benzaldehyde, dimethyl trisulfide, 2-nonanone, nonanal, decanal, 2-undecanone, indole, and 2-tridecanone) were prepared from internal standards in water. The headspace gases were then analyzed using microAnalyzer™, a miniaturized Gas Chromatography-Differential Mobility Spectrometry (GC-DMS) system. Differential mobility spectra showed monomer peaks of the analytes with the observed retention times below 3 min. Dispersion voltages, determining the dependence of signal intensities and compensation voltages of each analyte on the applied radio frequency (RF) voltages, were recorded for positive and negative modes. It was found that at increasing RF voltages the monomer peaks of compounds of lower molecular masses tend to shift to more negative compensation voltages; whereas, those of higher molecular masses tend to shift to more positive compensation voltages. The shift results in increase separation among each peak. The signal intensities for all peaks, however, tend to decrease at higher RF voltages. The RF voltage which gave optimum peaks separation and signal resolution was 1,200 V (corresponds to 24 kV/cm). This study demonstrates the potential of microAnalyzer™ as an integrated gas chromatograph detector for a preliminary screening system in the on-site detection of E. coli in water. The operational principle of the instrument is also presented in this study.
机译：大肠杆菌的12种已知挥发性代谢物的混合物（2,5-二甲基四氢呋喃，二甲基二硫键，2-庚酮，2,5-二甲基吡嗪，苯甲醛，二甲基三硫化物，2-壬酮，壬醛，癸醛，2-十一烷酮，吲哚和2 -三苯甲酮）是根据水中的内标制备的。然后使用microAnalyzer™（微型气相色谱-差动迁移谱法（GC-DMS）系统）分析顶空气体。差分迁移谱显示分析物的单体峰，观察到的保留时间低于3分钟。记录用于确定正负模式的色散电压，以确定每种分析物的信号强度和补偿电压对所施加的射频（RF）电压的依赖性。已经发现，在射频电压增加时，分子量较低的化合物的单体峰趋向于移向更多的负补偿电压。相反，分子量较高的分子倾向于转移到更正的补偿电压。该移动导致每个峰之间的分离度增加。但是，所有峰值的信号强度在较高的RF电压下往往会降低。提供最佳峰分离和信号分辨率的RF电压为1,200 V（相当于24 kV / cm）。这项研究证明了microAnalyzer™作为集成气相色谱检测仪的潜力，可用于现场筛查水中大肠杆菌的初步筛选系统。这项研究还介绍了仪器的操作原理。

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