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Targeted sampling of cementum for recovery of nuclear DNA from human teeth and the impact of common decontamination measures

机译:有针对性的牙骨质采样以从人的牙齿中回收核DNA以及常见净化方法的影响

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Background Teeth are a valuable source of DNA for identification of fragmented and degraded human remains. While the value of dental pulp as a source of DNA is well established, the quantity and presentation of DNA in the hard dental tissues has not been extensively studied. Without this knowledge common decontamination, sampling and DNA extraction techniques may be suboptimal. Targeted sampling of specific dental tissues could maximise DNA profiling success, while minimising the need for laborious sampling protocols and DNA extraction techniques, thus improving workflows and efficiencies. We aimed to determine the location of cellular DNA in non-degraded human teeth to quantify the yield of nuclear DNA from cementum, the most accessible and easily sampled dental tissue, and to investigate the effect of a common decontamination method, treatment with sodium hypochlorite (bleach).
机译:背景技术牙齿是DNA的宝贵来源,可用于鉴定碎片化和降解的人类遗骸。虽然牙髓作为DNA来源的价值已得到公认,但尚未对硬牙组织中DNA的数量和表现进行广泛研究。没有这种知识,常见的去污,采样和DNA提取技术可能不够理想。特定牙齿组织的目标采样可以最大程度地提高DNA谱图的成功率,同时最大程度地减少对费力的采样协议和DNA提取技术的需求,从而改善工作流程和效率。我们旨在确定细胞DNA在未降解的人类牙齿中的位置,以量化牙骨质(最易接近和易于采样的牙组织)中核DNA的产量,并研究使用次氯酸钠处理普通去污方法的效果(漂白)。

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