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首页> 外文期刊>Journal of bacteriology >Isolation of temperature-sensitive pantothenate kinase mutants of Salmonella typhimurium and mapping of the coaA gene.
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Isolation of temperature-sensitive pantothenate kinase mutants of Salmonella typhimurium and mapping of the coaA gene.

机译:Salmonella Typhimurium的温度敏感泛酸激酶突变体的分离及COAA基因的映射。

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摘要

Temperature-sensitive pantothenate kinase mutants of Salmonella typhimurium LT2 were selected by using the excretion of pantothenate at the nonpermissive temperature as a screening method. Thermolability of the pathothenate kinase activity in extracts of the mutants was demonstrated. The mutations were mapped at min 89 of the Salmonella chromosome, near rpoB, by transduction. As pantothenate kinase catalyzes the first step in the biosynthesis of coenzyme A from pantothenate, the new genetic locus has been designated coaA.
机译:通过使用在非智能温度下的泛酸盐作为筛选方法,选择通过使用泛酸钠的排泄来选择沙门氏菌的温度敏感的肝细胞激酶突变体。证明了突变体提取物中的病例激酶活性的热性。通过转导在沙门氏菌染色体的MIN 89下映射突变,通过转导。随着泛氢化激酶催化辅酶A从泛酸的第一步,新的遗传基因座已被指定为COAA。

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