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首页> 外文期刊>Journal of Experimental Botany >Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes
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Global gene expression analysis of transgenic, mannitol-producing, and salt-tolerant Arabidopsis thaliana indicates widespread changes in abiotic and biotic stress-related genes

机译：转基因，甘露醇生产和耐盐拟南芥的全球基因表达分析表明非生物和生物胁迫相关基因的广泛变化

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Mannitol is a putative osmoprotectant contributing to salt tolerance in several species. Arabidopsis plants transformed with the mannose-6-phosphate reductase (M6PR) gene from celery were dramatically more salt tolerant (at 100 mM NaCl) as exhibited by reduced salt injury, less inhibition of vegetative growth, and increased seed production relative to the wild type (WT). When treated with 200 mM NaCl, transformants produced no seeds, but did bolt, and exhibited less chlorosisecrosis and greater survival and dry weights than the WT. Without salt there were no M6PR effects on growth or phenotype, but expression levels of 2272 genes were altered. Many fewer differences (1039) were observed between M6PR and WT plants in the presence of salt, suggesting that M6PR pre-conditioned the plants to stress. Previous work suggested that mannitol is an osmoprotectant, but mannitol levels are invariably quite low, perhaps inadequate for osmoprotectant effects. In this study, transcriptome analysis reveals that the M6PR transgene activated the downstream abscisic acid (ABA) pathway by up-regulation of ABA receptor genes (PYL4, PYL5, and PYL6) and down-regulation of protein phosphatase 2C genes (ABI1 and ABI2). In the M6PR transgenic lines there were also increases in transcripts related to redox and cell wall-strengthening pathways. These data indicate that mannitol-enhanced stress tolerance is due at least in part to increased expression of a variety of stress-inducible genes.
机译：甘露醇是一种推定的渗透保护剂，有助于几种物种的耐盐性。与野生型相比，通过减少盐害，减少对营养生长的抑制和增加种子产量表现出，来自芹菜的甘露糖-6-磷酸还原酶（M6PR）基因转化的拟南芥植物具有更高的耐盐性（在100 mM NaCl下） （WT）。当用200 mM NaCl处理时，与WT相比，转化子不产生种子，但产生螺栓，并显示出较少的萎黄/坏死，存活率和干重。没有盐，对生长或表型没有M6PR影响，但是2272个基因的表达水平发生了变化。在盐的存在下，M6PR和WT植物之间观察到的差异更少（1039），这表明M6PR可以使植物预先适应胁迫。先前的工作表明甘露醇是一种渗透保护剂，但是甘露醇的含量总是很低，可能不足以起到渗透保护作用。在这项研究中，转录组分析显示，M6PR转基因通过上调ABA受体基因（PYL4，PYL5和PYL6）和下调蛋白磷酸酶2C基因（ABI1和ABI2）激活了下游脱落酸（ABA）途径。 。在M6PR转基因品系中，与氧化还原和细胞壁强化途径有关的转录本也有所增加。这些数据表明，甘露醇增强的应激耐受性至少部分归因于多种应激诱导基因的表达增加。

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