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首页> 外文期刊>Journal of the Institute of Brewing >Beer enemy number one: genetic diversity, physiology and biofilm formation of Lactobacillus brevis
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Beer enemy number one: genetic diversity, physiology and biofilm formation of Lactobacillus brevis

机译：啤酒敌人第一：遗传多样性，生理学和生物膜形成乳酸杆菌Brevis

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Lactobacillus brevis is the most significant beer spoilage bacteria worldwide. It is found as a contaminant at all stages of brewing, including during primary and secondary fermentation, storage, filtration and the packaging process. In production with flash pasteurisation and subsequent hygienic filling, avoiding and tracing secondary contaminations is the key to a microbiologically stable product. However, L. brevis strains vary in their spoilage potential and can grow in many different beer types. This study presents a physiological test scheme for growth potential and biofilm formation in various media. It was determined that a large number of L. brevis strains can form biofilms as a first coloniser. The identification of the species alone is therefore not enough to be sure of the spoilage risk, which shows the need for a more in depth differentiation. DNA fingerprint techniques are crucial to differentiate isolates of this species at strain level. The rep-PCR fingerprint system (GTG)(5) was used to differentiate a selected collection of 20 isolates, which were characterised in growth and biofilm formation in various media. The data showed a high variation within the selected isolates. As second step, generated fingerprint clusters of L. brevis were traced back to contamination sources in a German brewery, revealing a high number of isolates with potentially varying growth, spoilage and biofilm potential. L. brevis being the demonstrator species, the PCR system used is a powerful and compatible tracing and troubleshooting tool for all kinds of spoilage bacteria in the brewing industry. (C) 2019 The Institute of Brewing & Distilling
机译：Lactobacillus Brevis是全球最重要的啤酒腐败细菌。它被发现作为酿造的所有阶段的污染物，包括在初级和二次发酵，储存，过滤和包装过程中。在用闪蒸杀菌和随后的卫生填充的生产中，避免和跟踪二次污染是微生物稳定产品的关键。然而，L.Brevis菌株在腐败潜力中变化，并且可以在许多不同的啤酒类型中生长。本研究提出了各种培养基中生长潜力和生物膜形成的生理学测试方案。确定大量L.Brevis菌株可以将生物膜作为第一结肠机构形成。因此，单独的物种的鉴定不足以确保腐败的风险，这表明需要更深入分化的需求。 DNA指纹技术对于在应变水平下区分该物种的分离物至关重要。 REP-PCR指纹系统（GTG）（5）用于区分所选的20个分离物的收集，其特征在于各种培养基中的生长和生物膜形成。该数据在所选分离株内显示出高变化。作为第二步，L. Brevis的生成指纹簇被追溯到德国啤酒厂的污染源，揭示​​了大量的分离物，具有潜在不同的生长，腐败和生物膜潜力。 L. Brevis是演示品种，所使用的PCR系统是酿造行业各种腐败细菌的强大兼容的跟踪和故障排除工具。 （c）2019年酿造和蒸馏学院

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