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Quantification of strains in mixed lager yeast cultures using microsatellite PCR and GeXP

机译：使用微卫星PCR和GEXP的混合烘焙酵母培养物中菌株的定量

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摘要

Mixed culture fermentation with lager yeast is relatively new in brewing. Maintaining the proportion of strains during fermentation is important to assure the quality of beer and accordingly needs to be monitored. The current discriminatory methods have limited application for lager strains. In the present study, a rapid method to monitor the strain proportion of strains in mixed samples of two different lager yeast strains was developed using microsatellite polymerase chain reaction (PCR) and GeXP Genetic Analysis System. Microsatellite markers in the lager yeast genome were searched using SSR Hunter software. Quantitative PCR using these microsatellite markers was performed and PCR products were analysed by capillary electrophoresis. One locus - mitochondrial camitine acetyltransferase - was found to discriminate the two strains. A standard curve was generated based on the premixed samples of the two strains enabling the proportion of the stains to be determined. This method is a fast and reliable approach to monitor the strain proportion of mixed lager yeast fermentation. (C) 2020 The Institute of Brewing & Distilling
机译：啤酒酵母的混合培养发酵在酿造中相对较新。保持在发酵过程中菌株的比例对于确保啤酒的质量，因此需要监测啤酒。目前的歧视方法对贮藏菌株有限。在本研究中，使用微卫星聚合酶链反应（PCR）和GEXP遗传分析系统，开发了一种快速监测两种不同贮藏株的混合样品中应变比例的菌株的应变比例。使用SSR Hunter软件搜索贮藏酵母基因组中的微卫星标记。使用这些微卫星标记的定量PCR进行，并通过毛细管电泳分析PCR产物。发现一个基因座 - 线粒体阴影乙酰转移酶 - 发现两个菌株。基于两个菌株的预混合样品产生标准曲线，其能够确定污渍的比例。该方法是一种快速可靠的方法来监测混合贮藏酵母发酵的应变比例。 （c）2020酿造和蒸馏研究所

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  • 来源
    《Journal of the Institute of Brewing》 |2020年第2期|155-160|共6页
  • 作者

    Hou Xiaoping; Chen Lu; Yin Hua; Dong Jianjun; Yu Junhong; He Yang; Yang Mei;

  • 作者单位

    Tsingtao Brewery Co Ltd State Key Lab Biol Fermentat Engn Beer Tailiu Rd 602 Gate 3 Qingdao 266100 Shandong Peoples R China;

    Tsingtao Brewery Co Ltd State Key Lab Biol Fermentat Engn Beer Tailiu Rd 602 Gate 3 Qingdao 266100 Shandong Peoples R China;

    Tsingtao Brewery Co Ltd State Key Lab Biol Fermentat Engn Beer Tailiu Rd 602 Gate 3 Qingdao 266100 Shandong Peoples R China;

    Tsingtao Brewery Co Ltd State Key Lab Biol Fermentat Engn Beer Tailiu Rd 602 Gate 3 Qingdao 266100 Shandong Peoples R China;

    Tsingtao Brewery Co Ltd State Key Lab Biol Fermentat Engn Beer Tailiu Rd 602 Gate 3 Qingdao 266100 Shandong Peoples R China;

    Tsingtao Brewery Co Ltd State Key Lab Biol Fermentat Engn Beer Tailiu Rd 602 Gate 3 Qingdao 266100 Shandong Peoples R China;

    Tsingtao Brewery Co Ltd State Key Lab Biol Fermentat Engn Beer Tailiu Rd 602 Gate 3 Qingdao 266100 Shandong Peoples R China;

  • 收录信息 美国《科学引文索引》(SCI);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Saccharomyces pastorianus; mixed culture fermentation; strain proportion; Microsatellite PCR; GeXP genetic analysis system;

    机译：酿酒酵母;混合培养发酵;应变比例;微卫星PCR;GEXP遗传分析系统;

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