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首页> 外文期刊>Journal of the Institute of Brewing >Determination of Prolamins in Beers by ELISA and SDS-PACE
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Determination of Prolamins in Beers by ELISA and SDS-PACE

机译：ELISA和SDS-PACE测定啤酒中的醇溶蛋白

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Coeliac disease is triggered by exposure to the prolamin protein fraction of wheat, barley, or rye. The prolamin content of five lager beers and one wheat beer were analyzed by sodium do-decyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and immunoblotting and seven lager beers and three wheat beers were analyzed by enzyme-linked immunosorbent assay (ELISA). Most of the lager beers were made from barley and some had varying amounts of rice or corn as adjuncts. One of the beers was "gluten-free", having been produced from corn and buckwheat without barley. The lager beer samples were gel-filtered before ELISA or SDS-PAGE analysis. Prolamin proteins were found in all but one beer which was made of corn, rice and barley and which was not the "gluten-tree" beer. ELISA analysis was done using a commercially available gluten assay kit. For lager beers, a barley prolamin standard for ELISA was propanol-extracted from barley malt instead of using the prolamin standard of the gluten assay kit. As expected, the wheat beers contained much higher amounts of prolamins than the lager beers. The samples were studied by SDS-PAGE to identify different prolamin fractions. Proteins having a relative molecular mass in the range of 8000-17,000 and 38,000 and above were detected in immunoblotting by the prolamin sensitive antibody in the lager beers.
机译：暴露于小麦，大麦或黑麦的醇溶蛋白蛋白部分可引发腹腔疾病。通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）和免疫印迹法分析了5杯啤酒和1杯啤酒的醇溶蛋白含量，并用酶联免疫吸附法（ELISA）分析了7杯啤酒和3杯啤酒的醇溶蛋白。大部分啤酒是用大麦制成的，有些则用不同量的大米或玉米作为辅料。其中一种啤酒是“无麸质”的啤酒，由无大麦的玉米和荞麦制成。在ELISA或SDS-PAGE分析之前，将大啤酒样品进行凝胶过滤。在除了一种由玉米，大米和大麦制成的啤酒以外的所有啤酒中都发现了谷醇溶蛋白，该啤酒不是“面筋”啤酒。 ELISA分析使用市售的面筋测定试剂盒进行。对于大啤酒，从大麦麦芽中提取用于大麦醇溶酶的大麦醇溶蛋白标准品，而不使用面筋测定试剂盒的醇溶蛋白标准品。与预期的一样，小麦啤酒的醇溶蛋白含量比啤酒更大。通过SDS-PAGE研究样品，以鉴定不同的谷醇溶蛋白级分。通过啤酒中的醇溶蛋白敏感抗体在免疫印迹中检测到相对分子量在8000-17,000和38,000及更高范围内的蛋白质。

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