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首页> 外文期刊>Journal of Parasitology >DEVELOPMENT OF AN ANTIBODY-BASED CAPTURE ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTING ECHINOSTOMA CAPRONI (TREMATODA) IN EXPERIMENTALLY INFECTED RATS: KINETICS OF COPROANTIGEN EXCRETION
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DEVELOPMENT OF AN ANTIBODY-BASED CAPTURE ENZYME-LINKED IMMUNOSORBENT ASSAY FOR DETECTING ECHINOSTOMA CAPRONI (TREMATODA) IN EXPERIMENTALLY INFECTED RATS: KINETICS OF COPROANTIGEN EXCRETION

机译：基于抗体的捕获酶联免疫吸附测定法在实验感染大鼠中检测鹿角形衣原体（TREMATODA）的开发：辅助蛋白分泌的动力学

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The present study reports on the development of a coproantigen capture enzyme-linked immunosorbent assay (ELISA) for detecting Echinostoma caproni in experimentally infected rats. The capture ELISA was based on polyclonal rabbit antibodies that recognize excretory–secretory (ES) antigens. The detection limit of pure ES was 3 ng/ml in sample buffer and 60 ng/ml in fecal samples. The test was evaluated using a follow-up of 10 rats experimentally infected with 100 metacercariae of E. caproni, and the results were compared with those of other diagnostic methods such as parasitological examination and antibody titers determined by indirect ELISA. Coproantigens were detected in all the infected rats from the first day postinfection (DPI). The period of maximal coproantigen excretion was between 7 and 21 DPI. The values remained positive until 49–56 DPI, coinciding with the disappearance of the eggs in the stool samples of the infected rats. The kinetics of coproantigen detection were correlated with those of egg output. The present assay provides an alternative tool for the diagnosis of the echinostome infections. The proposed capture ELISA makes possible an earlier diagnosis than that provided by parasitological examination and indirect ELISA and also allows for the differentiation of past and current infections. Our results show that this assay can also be used to monitor the course of echinostome infections.
机译：本研究报告了一种用于检测实验感染大鼠中的棘皮E虫的辅助抗原捕获酶联免疫吸附试验（ELISA）的开发。捕获ELISA基于可识别排泄-分泌（ES）抗原的多克隆兔抗体。纯ES的检出限在样品缓冲液中为3 ng / ml，在粪便样品中为60 ng / ml。该试验是通过对10只实验性感染100只caperi尾cer肠杆菌的大鼠进行跟踪评估，并将结果与​​其他诊断方法（如寄生虫学检查和通过间接ELISA确定的抗体滴度）进行比较。从感染后第一天（DPI）开始，在所有感染的大鼠中都检测到了原抗原。最大原抗原排泄时间为7至21 DPI。直到49-56 DPI为止，该值一直保持正值，这与被感染大鼠粪便样本中卵的消失有关。协原抗原检测的动力学与产卵的动力学相关。本测定法提供了用于诊断棘突口感染的替代工具。拟议的捕获ELISA可以比寄生虫学检查和间接ELISA提供更早的诊断，并且可以区分过去和现在的感染。我们的结果表明，该测定法还可用于监测棘突动物感染的过程。

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