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首页> 外文期刊>Limnology >Dominant bacterioplankton populations in the meromictic Lake Suigetsu as determined by denaturing gradient gel electrophoresis of 16S rRNA gene fragments
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Dominant bacterioplankton populations in the meromictic Lake Suigetsu as determined by denaturing gradient gel electrophoresis of 16S rRNA gene fragments

机译：通过变性梯度凝胶电泳对16S rRNA基因片段进行变性，确定了淡水湖Suigetsu中的主要浮游细菌种群

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摘要

Lake Suigetsu is a typical meromictic lake in Japan characterized by a permanent chemocline at a depth of between 3 and 8 m separating the oxic freshwater mixolimnion from anoxic saline sulfidogenic monimolimnion. Dominant bacterioplankton populations in Lake Suigetsu were investigated using PCR-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. The bacterial population was vertically stratified, and temporal shifts in the microbial communities were observed in both the oxic and anoxic layers of Lake Suigetsu during the sampling period. Several dominant DGGE bands were excised and sequenced. In the chemocline, green sulfur bacteria phylogenetically related to the genera Prosthecochloris, Pelodyctyon, and Chlorobium within the phylum Chlorobi were dominant; the colorless sulfur bacteria closely related to the genus Thiomicrospira were detected. These sulfur bacterial groups appear to be important in the biogeochemical cycling of sulfur and/or carbon in Lake Suigetsu. Bacterial sequences affiliated with the Bacteroidetes phylum were frequent among the dominant fragments in the DGGE profiles throughout the water column. Populations possessing a fermentative metabolism exist in Bacteroidetes, suggesting they may contribute to the degradation of organic matter in the anoxic environment of Lake Suigetsu.
机译：Suigetsu湖是日本典型的冰山湖泊，其特征是在3至8 m的深度存在永久的趋化索环，将有氧淡水混合寡聚体与无氧盐生硫化物莫来酰亚胺分离开来。使用16S rRNA基因片段的PCR变性梯度凝胶电泳（DGGE）研究了Suigetsu湖中主要的浮游细菌种群。细菌种群在垂直方向上分层，并且在采样期间在水湖的有氧层和无氧层中都观察到了微生物群落的时间变化。几个主要的DGGE频段被切除并测序。在趋化霉素中，与绿球藻属内的Prosthecochloris，Pelodyctyon和Chlorobium属系统发育相关的绿色硫细菌占主导地位。检测到与硫微螺菌属密切相关的无色硫细菌。这些硫细菌群在Suigetsu湖中的硫和/或碳的生物地球化学循环中似乎很重要。在整个水柱中，DGGE图谱中的优势片段中频繁存在与细菌门脉杆菌相关的细菌序列。拟杆菌属中存在具有发酵代谢的种群，这表明它们可能有助于水ig湖缺氧环境中有机物的降解。

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  • 来源
    《Limnology》 |2009年第1期|63-69|共7页
  • 作者

    Ryuji Kondo; Akie Nakagawa; Lisa Mochizuki; Kyoko Osawa; Yukiyasu Fujioka; Junki Butani;

  • 作者单位

    Department of Marine Bioscience Fukui Prefectural University Obama Fukui 917-0003 Japan;

    Department of Marine Bioscience Fukui Prefectural University Obama Fukui 917-0003 Japan;

    Department of Marine Bioscience Fukui Prefectural University Obama Fukui 917-0003 Japan;

    Department of Marine Bioscience Fukui Prefectural University Obama Fukui 917-0003 Japan;

    Department of Marine Bioscience Fukui Prefectural University Obama Fukui 917-0003 Japan;

    Department of Marine Bioscience Fukui Prefectural University Obama Fukui 917-0003 Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Denaturing gradient gel electrophoresis; Meromictic lake; Microbial diversity; 16S rDNA;

    机译：变性梯度凝胶电泳M色湖微生物多样性16S rDNA;

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