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首页> 外文期刊>Materials & design >Biocompatibility and physicochemical characteristics of poly(ε-caprolactone)/poly(lactide-co-glycolide)ano-hydroxyapatite composite scaffolds for bone tissue engineering
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Biocompatibility and physicochemical characteristics of poly(ε-caprolactone)/poly(lactide-co-glycolide)ano-hydroxyapatite composite scaffolds for bone tissue engineering

机译：骨组织工程用聚（ε-己内酯）/聚（丙交酯-共-乙交酯）/纳米羟基磷灰石复合支架的生物相容性和理化特性

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This paper reports a novel method to prepare PCL/PLGA/HA (w/w = 6/4/2) bone tissue scaffold through melt-blending and particle-leaching. The ultrastructural and physicochemical properties of the scaffolds were studied by SEM, FTIR, XRD and TGA. Its porous rate and average size were 75.74 +/- 1.21% and 179.07 +/- 0.75 mu m, tensile strength and compressive strength were 147 +/- 5 MPa and 47 +/- 2 MPa. The 24-week degradation rate was 32.31 +/- 1.93%. Human mesenchymal stem cells were cultured in the scaffolds for 14-21 days in vitro. The results indicate that scaffolds can promote hMSCs proliferation and osteogenesis by enhancing the expression of ALP. The expression of the bone-related genes Runx2, OPN, OCN, BMP-2, collagen I, integrin a1, integrin b1, and SLP was markedly upregulated, suggesting that this scaffold can promote hMSCs differentiation, proliferation and maturation to osteoblasts. In vivo experiments, the scaffolds were implanted in a rabbit skull-defect model. Micro X-ray 3D imaging, HE, and immunohistochemistry revealed that the scaffold materials are degradable and also display excellent biocompatibility, along with the capacity to induce bone regeneration. Therefore, PCL/PLGA/HA scaffold materials would be promising in the repair and regeneration of non-weight-bearing bones. (C) 2016 Elsevier Ltd. All rights reserved.
机译：本文报道了一种通过熔融共混和颗粒浸取制备PCL / PLGA / HA（w / w = 6/4/2）骨组织支架的新方法。通过SEM，FTIR，XRD和TGA研究了支架的超微结构和理化性质。其多孔率和平均尺寸为75.74 +/- 1.21％和179.07 +/-0.75μm，抗张强度和抗压强度为147 +/- 5MPa和47 +/- 2MPa。 24周降解率为32.31 +/- 1.93％。将人间充质干细胞在支架中体外培养14-21天。结果表明支架可以通过增强ALP的表达来促进hMSCs的增殖和成骨作用。骨骼相关基因Runx2，OPN，OCN，BMP-2，胶原蛋白I，整联蛋白a1，整联蛋白b1和SLP的表达明显上调，表明该支架可以促进hMSCs向成骨细胞分化，增殖和成熟。在体内实验中，将支架植入兔子颅骨缺损模型中。 Micro X射线3D成像，HE和免疫组织化学分析显示，支架材料可降解，并且还具有出色的生物相容性以及诱导骨骼再生的能力。因此，PCL / PLGA / HA支架材料在非承重骨骼的修复和再生中将很有希望。 （C）2016 Elsevier Ltd.保留所有权利。

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