...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Materials science & engineering >Preparation of biocompatible molecularly imprinted film on biowaste-derived magnetic pomegranate rind carbon for protein recognition in biological sample
【24h】

Preparation of biocompatible molecularly imprinted film on biowaste-derived magnetic pomegranate rind carbon for protein recognition in biological sample

机译：生物吸入源石榴外皮碳生物相容性分子印迹薄膜的制备在生物样品中蛋白质识别

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Nowadays, the research on the recognition and separation of proteins has attracted extensive attention in the fields of materials science, bioengineering and life science. Protein imprinted polymers are ideal recognition materials, due to its high selectivity, good stability, easy preparation, and low cost. Herein, novel surface imprinting biowaste-derived molecularly imprinted polymers (BD-MIPs) were synthesized for specific recognition and purification of lysozyme (Lyz). This is the first time that magnetic pomegranate rind-derived carbon was used as a carrier to immobilize Lyz. Then, with the self-polymerization of dopamine, a large number of biocompatible recognition sites were generated under mild conditions. The physical/chemical properties and surface morphologies of the synthetic BD-MIPs were characterized, indicating that the imprinted film was successfully synthesized, and the BD-MIPs had good thermal stability and magnetic property. To investigate the recognition performance of BD-MIPs, four adsorption experiments were performed. The results show that BD-MIPs had a high adsorption capacity of 301.87 mg g(-1), fast equilibrium time within 40 min, satisfactory selectivity and good reusability for Lyz. Furthermore, the practicability of BD-MIPs was confirmed by the isolation of Lyz from a biological sample. The good adsorption capacity and gentle one-step preparation make the BD-MIPs attractive for Lyz recognition, which shows potential values in basic biomedical research, industrial protein purification and clinical diagnostics.
机译：如今，蛋白质识别和分离的研究引起了材料科学，生物工程和生命科学领域的广泛关注。蛋白质印迹聚合物是理想的识别材料，由于其高选择性，良好的稳定性，易于制备和低成本。在此，合成了新型表面积衍生的分子印迹聚合物（BD-MIPS）以进行溶菌酶（LYZ）的特异性识别和纯化。这是第一次使用磁石榴外皮衍生的碳作为固定液体的载体。然后，随着多巴胺的自聚合，在温和条件下产生大量的生物相容性识别位点。表征合成BD-MIPS的物理/化学性质和表面形态，表明印迹薄膜已成功合成，BD-MIPS具有良好的热稳定性和磁性。为了研究BD-MIPS的识别性能，进行了四种吸附实验。结果表明，BD-MIPS的吸附容量高301.87mg g（-1），40分钟内快速平衡时间，令人满意的选择性和良好的LYZ可重用性。此外，通过从生物样品中分离含量来证实BD-MIPS的实用性。良好的吸附能力和温和的一步准备使BD-MIPS对Lyz识别具有吸引力，这表明了基础生物医学研究，工业蛋白质纯化和临床诊断中的潜在价值。

著录项

  • 来源
    《Materials science & engineering》 |2020年第10期|111063.1-111063.9|共9页
  • 作者

    Xu Xinya; Guo Pengqi; Shu Hua; Qu Kenan; Ge Yanhui; Chen Guoning; Luo Zhimin; Du Wei; Chang Chun; Liu Ruilin; Fu Qiang;

  • 作者单位

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China|Shaanxi Univ Chinese Med Dept Pharmacol Xian 712046 Peoples R China;

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China;

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China;

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China;

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China;

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China;

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China;

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China;

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China;

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China;

    Xi An Jiao Tong Univ Sch Pharm 76 Yanta West Rd Xian 710061 Shaanxi Peoples R China;

  • 收录信息
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Protein recognition; Magnetic pomegranate rind carbon; Protein imprinted polymers; Agricultural waste; Biological application;

    机译：蛋白质识别;磁石榴外皮碳;蛋白质印迹聚合物;农业废物;生物应用;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号