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首页> 外文期刊>Journal of Microelectromechanical Systems >Microfluidic Systems Integrated With a Sample Pretreatment Device for Fast Nucleic-Acid Amplification
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Microfluidic Systems Integrated With a Sample Pretreatment Device for Fast Nucleic-Acid Amplification

机译：集成了样品预处理装置的微流控系统，可快速扩增核酸

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This paper presents a new miniature reverse-transcription polymerase chain-reaction (RT-PCR) system integrating a sample pretreatment device for fast nucleic-acid amplification and diagnosis of viruses and bacteria. In the system, a two-way serpentine-shape (s-shape) pneumatic micropump and a magnetic bioseparator were developed for separation and enrichment of viruses and bacteria. This new bioseparator can also be used as microheating chambers to perform RT-PCR. Taking advantage of the specific interaction between the antibodies on the surface of magnetic beads and the surface antigens on viruses or bacteria, the target virus and bacteria were recognized and further separated and purified from the biosamples by a magnetic field generated by the bioseparator. The target purified virus/bacteria was then lysed to release ribonucleic acid (RNA) or deoxyribonucleic acid (DNA) for the subsequent RT-PCR processes. Experimental results showed that the target virus/bacteria was successfully separated and enriched by the high specificity and selectivity of antibody-conjugated magnetic beads, and the subsequent amplification of RNA/DNA was automatically completed by utilizing the on-chip microheaters and the micro temperature sensor. The high mixing efficiency of the two-way s-shape pump and the rapid heating/cooling rate of the microheating chambers can significantly shorten the pretreatment and diagnosis processes. As a whole, the developed system may provide a powerful platform for sample pretreatment and fast disease diagnosis. $hfill$[2007-0089]
机译：本文介绍了一个新的微型逆转录聚合酶链反应（RT-PCR）系统，该系统集成了样品预处理设备，可快速进行核酸扩增并诊断病毒和细菌。在该系统中，开发了一种双向蛇形（s形）气动微型泵和磁性生物分离器，用于分离和富集病毒和细菌。这种新型生物分离器还可以用作微加热室，以进行RT-PCR。利用磁珠表面上的抗体与病毒或细菌上的表面抗原之间的特异性相互作用，可以识别靶病毒和细菌，并通过生物分离器产生的磁场从生物样品中进一步分离纯化。然后将目标纯化的病毒/细菌裂解，以释放核糖核酸（RNA）或脱氧核糖核酸（DNA），用于后续的RT-PCR过程。实验结果表明，通过结合抗体的磁珠的高特异性和选择性，成功分离并富集了目标病毒/细菌，并且利用片上微加热器和微温度传感器自动完成了随后的RNA / DNA扩增。 。双向S形泵的高混合效率和微加热腔室的快速加热/冷却速率可以显着缩短预处理和诊断过程。总体而言，开发的系统可以为样品预处理和快速疾病诊断提供强大的平台。 $ hfill $ [2007-0089]

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