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Effect of Fusarium graminearum and F. poae infection on Barley and Malt Quality

机译:禾本科镰刀菌和粉虱对大麦和麦芽品质的影响

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Objectives of this research were to investigate barley and malt quality as impacted by Fusarium graminearum and F.poae infection. F. graminearum is the primary pathogen responsible for the recent Fusarium Head Blight epidemics in the upper Midwestern USA, while F. poae is involved to a lesser extent. Inoculation and seed production in the greenhouse were utilized as a means of reducing the interfering effects of other microflora. Harvested seed from control and infected plants was micro-malted, and the malt quality of control and inoculated samples analyzed. Large amounts of deoxynivalenol,15-acetyl deoxy-nivalenol and zearalenone were present on samples inoculated with F. graminearum, while only small amounts of nivalenol were detected on the F.poae infected samples. The most noticeable effects of infection on barley quality were reduction in kernel plumpness and germination. In general the effects of F. graminearum on both barley and malt quality were more pronounced than those of F.poae. Reduction in kernel plumpness somewhat confounds the interpretation of malt quality results. However, infection with Fusarium did appear to have very pronounced effects on increasing wort soluble nitrogen, free amino nitrogen and wort color. These observations suggested increased proteolysis in the infected material. This is particularly true if one considers that up to 40% of kernels in the infected samples did not germinate, and would thus be expected to display reduced protease activity.
机译:这项研究的目的是调查大镰刀菌和禾谷镰刀菌感染对大麦和麦芽品质的影响。禾谷镰刀菌是导致美国中西部上半部镰刀菌最近流行的主要病原体,而禾本科镰刀菌的影响程度较小。温室中的接种和种子生产被用作减少其他微生物区系干扰作用的手段。将来自对照和受感染植物的收获种子微芽,并分析对照和接种样品的麦芽质量。接种禾本科镰刀菌的样品上存在大量的脱氧雪茄烯醇,15-乙酰基脱氧雪茄烯酚和玉米赤霉烯酮,而在受F.poae感染的样品中仅检测到少量的雪茄烯醇。感染对大麦品质最明显的影响是减少仁饱满度和发芽。一般而言,禾谷镰刀菌对大麦和麦芽品质的影响均比禾本科镰刀菌更明显。籽粒饱满度的降低在某种程度上混淆了麦芽品质结果的解释。但是,镰刀菌感染确实对增加麦芽汁可溶性氮,游离氨基氮和麦芽汁颜色有非常显着的影响。这些观察结果表明受感染物质中的蛋白水解增加。如果人们认为感染样品中多达40%的籽粒没有发芽,因此可以预期其蛋白酶活性降低,那就尤其如此。

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