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Theoretical Modeling and Experimental Validation of Surface Stress in Thrombin Aptasensor

机译:凝血酶适应性传感器表面应力的理论建模和实验验证

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Adsorption of target molecules on the immobilized microcantilever surface produced beam displacement due to the differential surface stress generated between the immobilized and non-immobilized surface. Surface stress is caused by the intermolecular forces between the molecules. Van der Waals, electrostatic forces, hydrogen bonding, hydrophobic effect and steric hindrance are some of the intermolecular forces involved. A theoretical framework describing the adsorption-induced microcantilever displacement is derived in this paper. Experimental displacement of thrombin aptamer-thrombin interactions was carried out. The relation between the electrostatic interactions involved between adsorbates (thrombin) as well as adsorbates and substrates (thrombin aptamer) and the microcantilever beam displacement utilizing the proposed mathematical model was quantified and compared to the experimental value. This exercise is important to aid the designers in microcantilever sensing performance optimization.
机译:靶分子在固定的微悬臂梁表面上的吸附由于固定的和非固定的表面之间产生的不同表面应力而产生束位移。表面应力是由分子之间的分子间力引起的。范德华力,静电力,氢键,疏水作用和位阻是其中涉及的一些分子间力。本文推导了描述吸附引起的微悬臂梁位移的理论框架。进行了凝血酶适体-凝血酶相互作用的实验置换。利用提出的数学模型对吸附物(凝血酶)以及吸附物和底物(凝血酶适体)之间涉及的静电相互作用与微悬臂梁位移之间的关系进行了量化,并与实验值进行了比较。此练习对于帮助设计人员优化微悬臂梁传感性能非常重要。

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