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Comparison of different methods to analyze a DNA computing library using the polymerase chain reaction

机译：比较使用聚合酶链反应分析DNA计算文库的不同方法

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摘要

Screening and analysis of collections of DNA molecules is a standard aspect of many DNA computing approaches. We describe the use of three different polymerase chain reaction (PCR) detection methods to screen specific members of a 5-site, 2-variable DNA computing library previously created using parallel overlap assembly of unique sequences generated from the SynDCode program. The three PCR methods (conventional gel-based PCR, SYBR Green real-time PCR and TaqMan real-time PCR) could all successfully identify individual library members separately or in a mixture. The TaqMan approach was also able to identify members in the original library we had not yet sequenced, providing more evidence supporting our hypothesis that the DNA library we generated may be complete. We expect these three approaches will be useful in future screening of other DNA computing libraries and structures.
机译：DNA分子集合的筛选和分析是许多DNA计算方法的标准方面。我们描述了使用三种不同的聚合酶链反应（PCR）检测方法来筛选5位，2变量DNA计算库的特定成员，该库先前使用从SynDCode程序生成的独特序列的平行重叠组装而创建。三种PCR方法（常规的基于凝胶的PCR，SYBR Green实时PCR和TaqMan实时PCR）都可以成功地分别或混合鉴定单个文库成员。 TaqMan方法还能够识别我们尚未测序的原始文库中的成员，提供了更多证据支持我们的假设，即我们生成的DNA文库可能是完整的。我们希望这三种方法在将来筛选其他DNA计算文库和结构时将很有用。

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  • 来源
    《Natural Computing》 |2012年第2期|p.339-349|共11页
  • 作者

    Cheryl P. Andam; Jonathan R. Driscoll; Jaime A. DiCesare; Tim N. Enke; Anthony J. Macula; Susannah Gal;

  • 作者单位

    Department of Biological Sciences, Binghamton University,Binghamton, NY 13902, USA,Department of Crop and Soil Science, Cornell University,Ithaca, NY 14850, USA;

    Department of Biological Sciences, Binghamton University,Binghamton, NY 13902, USA;

    Department of Biological Sciences, Binghamton University,Binghamton, NY 13902, USA;

    Department of Biological Sciences, Binghamton University,Binghamton, NY 13902, USA;

    Biomathematics Group, SUNY-Geneseo, Geneseo,NY 14454, USA;

    Department of Biological Sciences, Binghamton University,Binghamton, NY 13902, USA;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    DNA computing library; PCR; real-time PCR; SYBR green; taqman;

    机译：DNA计算文库;PCR;实时PCR;SYBR绿色;塔克曼;

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