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RNA polymerase I structure and transcription regulation

机译：RNA聚合酶I的结构和转录调控

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摘要

Transcription of ribosomal RNA by RNA polymerase (Pol) I initiates ribosome biogenesis and regulates eukaryotic cell growth. The crystal structure of Pol Ⅰ from the yeast Saccharomyces cerevisiae at 2.8 A resolution reveals all 14 subunits of the 590-kilodalton enzyme, and shows differences to Pol ⅠI. An 'expander' element occupies the DNA template site and stabilizes an expanded active centre cleft with an unwound bridge helix. A 'connector' element invades the cleft of an adjacent polymerase and stabilizes an inactive polymerase dimer. The connector and expander must detach during Pol Ⅰ activation to enable transcription initiation and cleft contraction by convergent movement of the polymerase 'core' and 'shelf' modules. Conversion between an inactive expanded and an active contracted polymerase state may generally underlie transcription. Regulatory factors can modulate the core-shelf interface that includes a 'composite' active site for RNA chain initiation, elongation, proofreading and termination.
机译：通过RNA聚合酶（Pol）I转录核糖体RNA可启动核糖体的生物发生并调节真核细胞的生长。酿酒酵母在2.8 A分辨率下的PolⅠ的晶体结构揭示了590千达尔顿酶的所有14个亚基，并显示出与PolⅠI的差异。 “扩展器”元件占据了DNA模板位点，并通过未缠绕的桥螺旋稳定了扩展的活动中心裂。 “连接子”元件侵入相邻聚合酶的缝隙并稳定无活性的聚合酶二聚体。在PolⅠ激活过程中，连接器和扩展子必须分离，以通过聚合酶“核心”和“架子”模块的聚合运动实现转录起始和裂隙收缩。在无活性的扩增和有活性的收缩聚合酶状态之间的转化通常可以作为转录的基础。调节因子可以调节核心-架子界面，其包括用于RNA链起始，延伸，校对和终止的“复合”活性位点。

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  • 来源
    《Nature》 |2013年第7473期|650-655|共6页
  • 作者

    Christoph Engel; Sarah Sainsbury; Alan C. Cheung; Dirk Kostrewa; Patrick Cramer;

  • 作者单位

    Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universitat Muenchen, Feodor-Lynen-Str. 25,81377 Munich, Germany;

    Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universitat Muenchen, Feodor-Lynen-Str. 25,81377 Munich, Germany;

    Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universitat Muenchen, Feodor-Lynen-Str. 25,81377 Munich, Germany;

    Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universitat Muenchen, Feodor-Lynen-Str. 25,81377 Munich, Germany;

    Gene Center and Department of Biochemistry, Center for Integrated Protein Science Munich (CIPSM), Ludwig-Maximilians-Universitat Muenchen, Feodor-Lynen-Str. 25,81377 Munich, Germany;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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