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首页> 外文期刊>Nature >The conserved protein DCN-1/Dcn1p is required for cullin neddylation in C-elegans and S-cerevisiae
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The conserved protein DCN-1/Dcn1p is required for cullin neddylation in C-elegans and S-cerevisiae

机译：保守蛋白DCN-1 / Dcn1p是C-线虫和S-酿酒酵母中cullin的Neddylation所需的

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摘要

SCF-type E3 ubiquitin ligases are multi-protein complexes required for polyubiquitination and subsequent degradation of target proteins by the 26S proteasome(1). Cullins, together with the RING-finger protein Rbx1, form the catalytic core of the ligase, and recruit the substrate-recognition module(1-4). Cycles of covalent modification of cullins by the ubiquitin-like molecule Nedd8 (neddylation)(5) and removal of Nedd8 by the COP9 signalosome (deneddylation) positively regulate E3 ligase activity(6,7). Here we report the identification and analysis of a widely conserved protein that is required for cullin neddylation in the nematode Caenorhabditis elegans and the yeast Saccharomyces cerevisiae. C. elegans DCN-1 and S. cerevisiae Dcn1p ( defective in cullin neddylation) are characterized by a novel UBA-like ubiquitin-binding domain and a DUF298 domain of unknown function. Consistent with their requirements for neddylation, DCN-1 and Dcn1p directly bind Nedd8 and physically associate with cullins in both species. Moreover, overexpression of Dcn1p in yeast results in the accumulation of Nedd8-modified cullin Cdc53p. Both in vivo and in vitro experiments indicate that Dcn1p does not inhibit deneddylation of Cdc53p by the COP9 signalosome, but greatly increases the kinetics of the neddylation reaction.
机译：SCF型E3泛素连接酶是26S蛋白酶体多泛素化和随后降解目标蛋白所需的多蛋白复合物（1）。 Cullins与RING手指蛋白Rbx1一起形成连接酶的催化核心，并募集底物识别模块（1-4）。遍在蛋白样分子Nedd8（凝结作用）（5）对cullins进行共价修饰的周期和COP9信号体（凝结作用）去除Nedd8的周期正调控E3连接酶的活性（6,7）。在这里，我们报告鉴定和分析的线虫秀丽隐杆线虫和酵母酿酒酵母中的cullin neddylation所需的广泛保守的蛋白质。秀丽隐杆线虫DCN-1和酿酒酵母Dcn1p（在cullin乙二醛化中有缺陷）的特征在于新颖的UBA样泛素结合结构域和功能未知的DUF298结构域。符合其对糊化作用的要求，DCN-1和Dcn1p直接结合Nedd8，并与这两种物种的cullins物理结合。此外，Dcn1p在酵母中的过表达导致Nedd8修饰的cullin Cdc53p的积累。体内和体外实验均表明，Dcn1p不会抑制COP9信号小体对Cdc53p的树突化作用，但会大大增加树酯化反应的动力学。

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