• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Nature >A highly active synthetic mammalian retrotransposon
【24h】

A highly active synthetic mammalian retrotransposon

机译：高度活跃的合成哺乳动物反转录转座子

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

LINE-1 (L1) elements are retrotransposons that comprise large fractions of mammalian genomes(1). Transcription through L1 open reading frames is inefficient owing to an elongation defect(2), inhibiting the robust expression of L1 RNA and proteins, the substrate and enzyme(s) for retrotransposition(3-5). This elongation defect probably controls L1 transposition frequency in mammalian cells. Here we report bypassing this transcriptional defect by synthesizing the open reading frames of L1 from synthetic oligonucleotides, altering 24% of the nucleic acid sequence without changing the amino acid sequence. Such resynthesis led to greatly enhanced steady-state L1 RNA and protein levels. Remarkably, when the synthetic open reading frames were substituted for the wild-type open reading frames in an established retrotransposition assay(4), transposition levels increased more than 200-fold. This indicates that there are probably no large, rigidly conserved cis-acting nucleic acid sequences required for retrotransposition within L1 coding regions. These synthetic retrotransposons are also the most highly active L1 elements known so far and have potential as practical tools for manipulating mammalian genomes.
机译：LINE-1（L1）元件是反转录转座子，包含大部分哺乳动物基因组（1）。由于延伸缺陷（2），通过L1开放阅读框的转录效率很低（2），抑制了L1 RNA和蛋白质，底物和酶逆转录的强健表达（3-5）。这种伸长缺陷可能控制了哺乳动物细胞中的L1转座频率。在这里我们报告通过从合成的寡核苷酸合成L1的开放阅读框来绕过这种转录缺陷，改变了24％的核酸序列而没有改变氨基酸序列。这种重新合成导致大大提高了稳态L1 RNA和蛋白质水平。值得注意的是，在已建立的逆转座分析中，当用合成的开放阅读框代替野生型开放阅读框时，转座水平增加了200倍以上。这表明在L1编码区内逆转座可能不需要大的，严格保守的顺式作用核酸序列。这些合成的反转录转座子也是迄今为止已知的活性最高的L1元件，具有操纵哺乳动物基因组的实用工具的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号