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首页> 外文期刊>Nature >The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity
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The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity

机译：钙渗透通道OSCA1.3调节植物气孔免疫力

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摘要

Perception of biotic and abiotic stresses often leads to stomatal closure in plants(1,2). Rapid influx of calcium ions (Ca2+) across the plasma membrane has an important role in this response, but the identity of the Ca(2+)channels involved has remained elusive(3,4). Here we report that theArabidopsis thalianaCa(2+)-permeable channel OSCA1.3 controls stomatal closure during immune signalling. OSCA1.3 is rapidly phosphorylated upon perception of pathogen-associated molecular patterns (PAMPs). Biochemical and quantitative phosphoproteomics analyses reveal that the immune receptor-associated cytosolic kinase BIK1 interacts with and phosphorylates the N-terminal cytosolic loop of OSCA1.3 within minutes of treatment with the peptidic PAMP flg22, which is derived from bacterial flagellin. Genetic and electrophysiological data reveal that OSCA1.3 is permeable to Ca2+, and that BIK1-mediated phosphorylation on its N terminus increases this channel activity. Notably, OSCA1.3 and its phosphorylation by BIK1 are critical for stomatal closure during immune signalling, and OSCA1.3 does not regulate stomatal closure upon perception of abscisic acid-a plant hormone associated with abiotic stresses. This study thus identifies a plant Ca(2+)channel and its activation mechanisms underlying stomatal closure during immune signalling, and suggests specificity in Ca(2+)influx mechanisms in response to different stresses.A study inArabidopsis thalianashows that the immune receptor-associated cytosolic kinase BIK1 phosphorylates OSCA1.3 and identifies OSCA1.3 as the pathogen-responsive Ca2+-permeable channel that regulates stomatal closure.
机译：对生物和非生物应激的感知通常导致植物中的气孔闭合（1,2）。在血浆膜上的钙离子（Ca2 +）的快速流入在这种反应中具有重要作用，但涉及的Ca（2+）通道的身份仍然难以捉摸（3,4）。在这里，我们报告的是，在免疫信号传导期间，肌巴肌瘤（2 +）渗透通道OSCA1.3控制气孔闭合。在感知到病原体相关分子模式（PAMP）时，OSCA1.3迅速磷酸化。生物化学和定量磷蛋白酶分析表明，免疫受体相关的胞质激酶Bik1与肌肉蛋白蛋白的肽PAMP FLG22的逐次相互作用和磷酸化OSCA1.3的N-末端细胞骨环。遗传和电生理数据显示，OSCA1.3可渗透至Ca2 +，并且其N末端对介导的磷酸化增加了该通道活性。值得注意的是，奥斯卡1.3及其磷酸化对免疫信号传导期间的气孔闭合至关重要，并且OSCA1.3在对脱钙酸的感知 - 一种与非生物胁迫相关的植物激素时不调节气孔闭合。因此，该研究鉴定了免疫信号传导期间气孔闭合的植物Ca（2+）通道及其激活机制，并表明Ca（2+）流入机制的特异性响应于不同的应力。研究Inarabopsis Thalianis的免疫受体相关的细胞骨激酶Bik1磷酸化OSCA1.3并将OSCA1.3识别为调节气孔闭合的病原体响应性CA2 +可透视渠道。

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  • 来源
    《Nature》 |2020年第7826期|569-573|共5页
  • 作者

    Thor Kathrin; Jiang Shushu; Michard Erwan; George Jeoffrey; Scherzer Soenke; Huang Shouguang; Dindas Julian; Derbyshire Paul; Leitao Nuno; DeFalco Thomas A.; Koester Philipp; Hunter Kerri; Kimura Sachie; Gronnier Julien; Stransfeld Lena; Kadota Yasuhiro; Buecherl Christoph A.; Charpentier Myriam; Wrzaczek Michael; MacLean Daniel; Oldroyd Giles E. D.; Menke Frank L. H.; Roelfsema M. Rob G.; Hedrich Rainer; Feijo Jose; Zipfel Cyril;

  • 作者单位

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England;

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England|Chinese Acad Sci Shanghai Inst Biochem & Cell Biol Ctr Excellence Mol Cell Sci Shanghai Peoples R China;

    Univ Maryland Dept Cell Biol & Mol Genet College Pk MD 20742 USA;

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England|Univ Zurich Inst Plant & Microbial Biol Zurich Basel Plant Sci Ctr Zurich Switzerland;

    Univ Wurzburg Dept Mol Plant Physiol & Biophys Wurzburg Germany;

    Univ Wurzburg Dept Mol Plant Physiol & Biophys Wurzburg Germany;

    Univ Zurich Inst Plant & Microbial Biol Zurich Basel Plant Sci Ctr Zurich Switzerland;

    John Innes Ctr Dept Cell & Dev Biol Norwich Res Pk Norwich Norfolk England|Synthace Ltd London England;

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England|Univ Zurich Inst Plant & Microbial Biol Zurich Basel Plant Sci Ctr Zurich Switzerland;

    Univ Zurich Inst Plant & Microbial Biol Zurich Basel Plant Sci Ctr Zurich Switzerland;

    Univ Helsinki Fac Biol & Environm Sci Viikki Plant Sci Ctr Organismal & Evolutionary Biol Res Programme VIPS Helsinki Finland;

    Univ Helsinki Fac Biol & Environm Sci Viikki Plant Sci Ctr Organismal & Evolutionary Biol Res Programme VIPS Helsinki Finland|Ritsumeikan Univ Ritsumeikan Global Innovat Res Org Shiga Japan;

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England|Univ Zurich Inst Plant & Microbial Biol Zurich Basel Plant Sci Ctr Zurich Switzerland;

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England|Univ Zurich Inst Plant & Microbial Biol Zurich Basel Plant Sci Ctr Zurich Switzerland;

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England|RIKEN Ctr Sustainable Resource Sci Plant Immun Res Grp Yokohama Kanagawa Japan;

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England|Dr Friedrich Eberth Arzneimittel GmbH Ursensollen Germany;

    John Innes Ctr Dept Cell & Dev Biol Norwich Res Pk Norwich Norfolk England;

    Univ Helsinki Fac Biol & Environm Sci Viikki Plant Sci Ctr Organismal & Evolutionary Biol Res Programme VIPS Helsinki Finland;

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England;

    John Innes Ctr Dept Cell & Dev Biol Norwich Res Pk Norwich Norfolk England|Univ Cambridge Sainsbury Lab Cambridge England;

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England;

    Univ Wurzburg Dept Mol Plant Physiol & Biophys Wurzburg Germany;

    Univ Wurzburg Dept Mol Plant Physiol & Biophys Wurzburg Germany;

    Univ Maryland Dept Cell Biol & Mol Genet College Pk MD 20742 USA;

    Univ East Anglia Sainsbury Lab Norwich Res Pk Norwich Norfolk England|Univ Zurich Inst Plant & Microbial Biol Zurich Basel Plant Sci Ctr Zurich Switzerland;

  • 收录信息 美国《科学引文索引》(SCI);美国《工程索引》(EI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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