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首页> 外文期刊>Nature >Suppression of auxin signal transduction by a MAPK cascade in higher plants.
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Suppression of auxin signal transduction by a MAPK cascade in higher plants.

机译：MAPK级联在高等植物中抑制生长素信号转导。

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摘要

The plant hormone auxin activates many early response genes that are thought to be responsible for diverse aspects of plant growth and development. It has been proposed that auxin signal transduction is mediated by a conserved signalling cascade consisting of three protein kinases: the mitogen-activated protein kinase (MAPK), MAPK kinase (MAPKK) and MAPKK kinase (MAPKKK). Here we show that a specific plant MAPKKK, NPK1, activates a MAPK cascade that leads to the suppression of early auxin response gene transcription. A mutation in the kinase domain abolishes NPK1 activity, and the presence of the carboxy-terminal domain diminishes the kinase activity. Moreover, the effects of NPK1 on the activation of a MAPK and the repression of early auxin response gene transcription are specifically eliminated by a MAPK phosphatase. Transgenic tobacco plants overexpressing the NPK1 kinase domain produced seeds defective in embryo and endosperm development. These results suggest that auxin sensitivity may be balanced by antagonistic signalling pathways that use a distinct MAPK cascade in higher plants.
机译：植物激素生长素激活许多早期反应基因，这些基因被认为与植物生长和发育的各个方面有关。已经提出，生长素信号转导是由保守的信号级联介导的，该保守的信号级联由三种蛋白激酶组成：有丝分裂原活化蛋白激酶（MAPK），MAPK激酶（MAPKK）和MAPKK激酶（MAPKKK）。在这里，我们显示特定的植物MAPKKK NPK1激活MAPK级联反应，从而导致早期生长素反应基因转录的抑制。激酶结构域中的突变消除了NPK1活性，而羧基末端结构域的存在则减弱了激酶活性。此外，NPK1对MAPK活化和早期生长素应答基因转录的抑制作用被MAPK磷酸酶特异性消除。过表达NPK1激酶结构域的转基因烟草植物产生的种子在胚芽和胚乳发育中存在缺陷。这些结果表明，在高等植物中，生长素敏感性可能与使用独特的MAPK级联反应的拮抗信号通路平衡。

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