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首页> 外文期刊>Photodiagnosis and Photodynamic Therapy >Enhancement of blood porphyrin emission intensity with aminolevulinic acid administration: A new concept for photodynamic diagnosis of early prostate cancer
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Enhancement of blood porphyrin emission intensity with aminolevulinic acid administration: A new concept for photodynamic diagnosis of early prostate cancer

机译：氨基乙酰丙酸增强血卟啉释放强度：早期前列腺癌光动力诊断的新概念

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Background: The objective of this paper was to verify if the oral administration of S-aminolevulinic acid (ALA) in animals with prostate tumor can increase the sensitivity of cancer diagnosis by protoporphyrin IX blood autofluorescence. In this study, the autofluorescence of blood porphyrin was analyzed using fluorescence spectroscopy on healthy male NUDE mice and in those with prostate cancer induced by the inoculation of DU145 cells. Methods: A total of 18 male NUDE mice, ~8 weeks old on arrival were divided into 3 groups:Control, Tumor and ALA Tumor. The autofluorescence of blood porphyrin of the groups was analyzed using fluorescence spectroscopy at different days after tumor induction, to monitor the tumor progression. Emission spectra were obtained by exciting the samples at 405 nm. The animals inoculated had their blood collected with and without oral ALA solution administration to compare PPIX endogenous (Tumor group) and exogenous (ALA Tumor group) signal intensity and to establish a method to diagnosis early prostate cancer. Results: Significant differences were observed in autofluorescence intensities measured in the 575-725 nm spectral regions for the studied groups. Conclusions: The results showed an enhancement of almost 100% in blood PPIX fluorescence,using the oral administration of 8-aminolevulinic acid on male NUDE mice with prostate cancer,making fluorescence measurements more accurate and sensitive since the first week after tumor induction.
机译：背景：本文的目的是验证在患有前列腺肿瘤的动物中口服S-氨基乙酰丙酸（ALA）是否可以提高原卟啉IX血液自发荧光对癌症诊断的敏感性。在这项研究中，使用荧光光谱法对健康的雄性NUDE小鼠和接种DU145细胞诱导的前列腺癌的小鼠进行了血卟啉的自发荧光分析。方法：将约18周龄的18只雄性NUDE雄性小鼠分为3组：对照组，肿瘤和ALA肿瘤。在肿瘤诱导后的不同天使用荧光光谱法分析各组血卟啉的自发荧光，以监测肿瘤的进展。通过在405 nm处激发样品获得发射光谱。接种的动物在有或没有口服ALA溶液的情况下采集血液，以比较PPIX内源性（肿瘤组）和外源性（ALA肿瘤组）信号强度，并建立诊断早期前列腺癌的方法。结果：在研究组的575-725 nm光谱区域中测得的自发荧光强度存在显着差异。结论：结果显示，口服8-氨基乙酰丙酸对雄性前列腺癌NUDE小鼠口服PPIX荧光后，其荧光强度几乎提高了100％，自诱导肿瘤后第一周以来，荧光测量结果更加准确，灵敏。

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