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Molecular cloning of ultraviolet-sensitive visual pigment in juvenile Champsocephalus gunnari (Channichthyidae)

机译:幼年型Champsocephalus gunnari(Channichthyidae)的紫外线敏感视觉色素的分子克隆

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摘要

We examined histologically the retinal cone photoreceptor mosaics of 0- to 6-year-old Champsocephalus gunnari. In the retina of 0- to 3-year-old fish, three types of cone cells, single-, double- and triple-cone, were identified. The triple-cone cells were localized near the optic papilla. In the outer region of the optic papilla, double-cone and single-cone cells were aligned alternately. Only double-cone cells were distributed in the peripheral retina. There were very few single-cone cells in the retinas of 4- to 6-year-old fish. The putative ultraviolet (UV)-sensitive visual pigment (SWS1) gene was isolated from the retina of 0- to 1-year-old fish. The recombinant opsin, encoded by this gene, showed a peak absorbance at 358 nm. It was considered that the UV sensitivity in juvenile C. gunnari might increase foraging efficiency by enhancing the contrast of the planktonic prey in the Antarctic summer.
机译:我们在组织学上检查了0至6岁的Champsocephalus gunnari的视网膜锥感光细胞镶嵌体。在0至3岁鱼的视网膜中,发现了三种圆锥细胞,即单圆锥,双圆锥和三圆锥。三锥细胞位于视神经乳头附近。在视神经乳头的外部区域,双锥体和单锥体细胞交替排列。仅双锥细胞分布在周围的视网膜中。 4至6岁鱼的视网膜中几乎没有单锥细胞。假定的紫外线(UV)敏感视觉色素(SWS1)基因是从0至1岁鱼的视网膜中分离出来的。由该基因编码的重组视蛋白在358nm处显示出峰吸收。有人认为,幼小C. gunnari的紫外线敏感性可能会通过增强南极夏季浮游生物的对比度来提高觅食效率。

著录项

  • 来源
    《Polar biology》 |2011年第2期|p.235-242|共8页
  • 作者单位

    Graduate School of Bioresources, Mie University, Tsu, Mie 514-8507, Japan;

    Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan;

    Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan;

    Tokyo Kasei-Gakuin University, Machida, Tokyo 194-0292, Japan;

    National Institute of Radiological Sciences, Inage, Chiba 263-8555, Japan;

    Graduate School of Frontier Sciences, The University of Tokyo, Kashiwa, Chiba 277-8562, Japan;

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  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    champsocephalus gunnari; ultraviolet-sensitive opsin; visual pigment; cDNA cloning;

    机译:头颅香菇紫外线敏感视蛋白视觉色素cDNA克隆;

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