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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >ELECTROPHORETIC VARIATION IN ADENYLATE KINASE OF NEISSERIA MENINGITIDIS IS DUE TO INTER- AND INTRASPECIES RECOMBINATION
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ELECTROPHORETIC VARIATION IN ADENYLATE KINASE OF NEISSERIA MENINGITIDIS IS DUE TO INTER- AND INTRASPECIES RECOMBINATION

机译：脑膜炎奈瑟球菌腺苷酸激酶的电泳变化是由于种间和种内重组引起的。

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In prokaryotic and eukaryotic organisms, the electrophoretic variation in housekeeping enzymes from natural populations is assumed to have arisen by the accumulation of stochastic predominantly neutral mutations, In the naturally transformable bacterium Neisseria meningitidis, we show that variation in the electrophoretic mobility of adenylate kinase is due to inter- and intraspecies recombination rather than mutation. The nucleotide sequences of the adenylate kinase gene (adk) from isolates that express the predominant slow electrophoretic variant were rather uniform, differing in sequence at an average of 1.1% of nucleotide sites. The adk sequences of rare isolates expressing the fast migrating variant were identical to each other but had a striking mosaic structure when compared to the adk genes from strains expressing the predominant variant. Thus the sequence from the fast variants was identical to those of typical slow variants in the first 158 bp of the gene but differed by 8.4% in the rest of the gene (nt 159-636). The fast electrophoretic variant appears to have arisen by the replacement of most of the meningococcal gene with the corresponding region from the adk gene of a closely related Neisseria species. The adk genes expressing the electrophoretic variant with intermediate mobility were perfect, or almost perfect, recombinants between the adk genes expressing the fast and slow variants. Recombination may, therefore, play a major role in the generation of electrophoretically detectable variation in housekeeping enzymes of some bacterial species. [References: 43]
机译：在原核和真核生物中，假定自然种群中管家酶的电泳变化是由随机的，主要是中性突变的积累引起的。在自然可转化的细菌脑膜炎奈瑟氏菌中，我们表明腺苷酸激酶的电泳迁移率变化是由于种间重组，而不是突变。表达主要的慢速电泳变异体的分离株的腺苷酸激酶基因（adk）的核苷酸序列相当一致，平均核苷酸位点的1.1％差异。与来自表达主要变异体的菌株的adk基因相比，表达快速迁移变异体的稀有分离株的adk序列彼此相同，但具有惊人的镶嵌结构。因此，来自快速变体的序列在基因的前158 bp中与典型的慢变体的序列相同，但在其余基因中差异8.4％（nt 159-636）。快速电泳变异似乎是通过用密切相关的奈瑟氏球菌属的adk基因的相应区域替换大部分脑膜炎球菌基因而产生的。表达具有中等迁移性的电泳变体的adk基因是表达快变体和慢变体的adk基因之间的完美或几乎完美的重组体。因此，重组可能在某些细菌种类的内务酶的电泳可检测变化的产生中起主要作用。 [参考：43]

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