Single-molecule microscopy of Cre recombination

Jeffrey P. Mumm

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Single-molecule microscopy of Cre recombination

机译：Cre重组的单分子显微镜

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The Cre recombination system, which has been commonly used as a bioengineering tool for altering eukaryotic genomes in a highly efficient and precise manner, is an archetypical member of a large family of tyrosine site-specific DNA recombinases. Different family members are responsible for a wide range of biological pathways such as regulation of gene expression and DNA replication, plasmid copy number maintenance, conjugative transposition, catenated circle resolution, daughter chromosome segregation, and prokaryotic telomere processing.

机译：Cre重组系统已广泛用作酪氨酸位点特异DNA重组酶家族的原型成员，而Cre重组系统通常用作高效，精确地改变真核生物基因组的生物工程工具。不同的家族成员负责广泛的生物学途径，例如基因表达和DNA复制的调控，质粒拷贝数的维持，共轭转座，链状环拆分，子染色体分离和原核端粒加工。

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《Proceedings of the National Academy of Sciences of the United States of America》 |2012年第51期|20779-20780|共2页
作者
Jeffrey P. Mumm;
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Department of Biochemistry, Brandeis University, Waltham, MA 02454;

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收录信息美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
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正文语种 eng
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专利

1. A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic neural progenitors A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic neural progenitors A Nestin-cre transgenic mouse is insufficient for recombination in early embryonic neural progenitors [J] . H. Troy Ghashghaei, Huixuan Liang, Simon Hippenmeyer Biology Open . 2012,第12期

机译：Nestin-cre转基因小鼠不足以在早期胚胎神经祖细胞中重组Nestin-cre转基因小鼠不足以在早期胚胎神经祖细胞中重组Nestin-cre转基因小鼠不足以在早期胚胎神经祖细胞中重组。
2. Capturing reaction paths and intermediates in Cre-loxP recombination using single-molecule fluorescence [J] . Justin N. M. Pinkney, Pawel Zawadzki, Jaroslaw Mazuryk, Proceedings of the National Academy of Sciences of the United States of America . 2012,第51期

机译：使用单分子荧光捕获Cre-loxP重组中的反应路径和中间体
3. Real-time single-molecule tethered particle motion experiments reveal the kinetics and mechanisms of Cre-mediated site-specific recombination. [J] . Fan HF Nucleic Acids Research . 2012,第13期

机译：实时单分子束缚粒子运动实验揭示了Cre介导的位点特异性重组的动力学和机理。
4. Application of the Cre-loxP Recombination System for Two ILV2 Alleles Disruption in an Industrial Brewer's Yeast Strain [C] . Jun Lu, Yefu Chen, Deguang Wu, International conference on applied biotechnology . 2014

机译：Cre-loxP重组系统在工业啤酒酵母菌株中两个ILV2等位基因突变的应用
5. Modes of DNA discrimination and specificity in Cre/LoxP site-specific recombination: Recognition of the LoxP spacer and binding elements. [D] . Gelato, Kathy Ann. 2007

机译：Cre / LoxP位点特异性重组中DNA区分和特异性的模式：LoxP间隔子和结合元件的识别。
6. Single-molecule microscopy of Cre recombination [O] . Jeffrey P. Mumm 2012

机译：Cre重组的单分子显微镜
7. Capturing reaction paths and intermediates in Cre-loxP recombination using single-molecule fluorescence. [O] . Pinkney, JN, Zawadzki, P, Mazuryk, J, 2012

机译：使用单分子荧光捕获Cre-loxP重组中的反应路径和中间体。

Single-molecule microscopy of Cre recombination

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