...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >RNA polymerase and transcription elongation factor Spt4/5 complex structure
【24h】

RNA polymerase and transcription elongation factor Spt4/5 complex structure

机译：RNA聚合酶和转录延伸因子Spt4 / 5复杂结构

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Spt4/5 in archaea and eukaryote and its bacterial homolog NusG is the only elongation factor conserved in all three domains of life and plays many key roles in cotranscriptional regulation and in recruiting other factors to the elongating RNA polymerase. Here, we present the crystal structure of Spt4/5 as well as the structure of RNA polymerase-Spt4/5 complex using cryoelectron microscopy reconstruction and single particle analysis. The Spt4/5 binds in the middle of RNA polymerase claw and encloses the DNA, reminiscent of the DNA polymerase clamp and ring helicases. The transcription elongation complex model reveals that the Spt4/5 is an upstream DNA holder and contacts the nontemplate DNA in the transcription bubble. These structures reveal that the cellular RNA polymerases also use a strategy of encircling DNA to enhance its processivity as commonly observed for many nucleic acid processing enzymes including DNA polymerases and helicases.
机译：古细菌和真核生物中的Spt4 / 5及其细菌同源物NusG是在生命的所有三个结构域中均保守的唯一延伸因子，并且在共转录调控和将其他因子募集至延伸RNA聚合酶中起着关键作用。在这里，我们介绍了Spt4 / 5的晶体结构以及RNA聚合酶-Spt4 / 5复合物的结构，采用了冷冻电子显微镜重建和单颗粒分析。 Spt4 / 5结合在RNA聚合酶爪的中间并包裹DNA，让人联想到DNA聚合酶钳和环解旋酶。转录延伸复合物模型显示，Spt4 / 5是上游DNA持有者，并与转录气泡中的非模板DNA接触。这些结构表明，细胞RNA聚合酶也采用包围DNA的策略来增强其合成能力，这是许多核酸加工酶（包括DNA聚合酶和解旋酶）通常都观察到的。

著录项

  • 来源
  • 作者

    Brianna J. Klein; Daniel Bose; Kevin J. Baker; Zahirah M. Yusoff; Xiaodong Zhang; Katsuhiko S. Murakami;

  • 作者单位

    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802;

    Division of Molecular Biosciences, Centre for Structural Biology, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, United Kingdom;

    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802;

    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802;

    Division of Molecular Biosciences, Centre for Structural Biology, Department of Life Sciences, Faculty of Natural Sciences, Imperial College London, London SW7 2AZ, United Kingdom;

    Department of Biochemistry and Molecular Biology, Pennsylvania State University, University Park, PA 16802;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cryo-EM; Spt4/5-DSIF-NusG; X-ray crystallography;

    机译：低温电磁Spt4 / 5-DSIF-NusG;X射线晶体学;

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号