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Structure of a mitochondrial ribosome with minimal RNA

机译：具有最小RNA的线粒体核糖体的结构

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摘要

The Leishmania tarentolae mitochondrial ribosome (Lmr) is a minimal ribosomal RNA (rRNA)-containing ribosome. We have obtained a cryo-EM map of the Lmr. The map reveals several features that have not been seen in previously-determined structures of eubacterial or eukaryotic (cytoplasmic or organel-lar) ribosomes to our knowledge. Comparisons of the Lmr map with X-ray crystallographic and cryo-EM maps of the eubacterial ribosomes and a cryo-EM map of the mammalian mitochondrial ribosome show that (ⅰ) the overall structure of the Lmr is considerably more porous, (ⅱ) the topology of the intersubunit space is significantly different, with fewer intersubunit bridges, but more tunnels, and (ⅲ) several of the functionally-important rRNA regions, including the a-sarcin-ricin loop, have different relative positions within the structure. Furthermore, the major portions of the mRNA channel, the tRNA passage, and the nascent polypeptide exit tunnel contain Lmr-specific proteins, suggesting that the mechanisms for mRNA recruitment, tRNA interaction, and exiting of the nascent polypeptide in Lmr must differ markedly from the mechanisms deduced for ribosomes in other organisms. Our study identifies certain structural features that are characteristic solely of mitochondrial ribosomes and other features that are characteristic of both mitochondrial and chloroplast ribosomes (i.e., organellar ribosomes).
机译：塔氏利什曼原虫线粒体核糖体（Lmr）是一种含有最小核糖体RNA（rRNA）的核糖体。我们已经获得了Lmr的低温电磁图。该图揭示了据我们所知，真细菌或真核（胞质或细胞器）核糖体的先前确定结构中未发现的几个特征。 Lmr图与真细菌核糖体的X射线晶体学图和冷冻EM图以及哺乳动物线粒体核糖体的冷冻EM图的比较显示（ⅰ）Lmr的整体结构明显更多孔，（ⅱ）亚基间空间的拓扑结构显着不同，亚基间桥较少，但隧道更多，并且（α）几个功能上重要的rRNA区域（包括a-sarcin-ricin环）在结构中具有不同的相对位置。此外，mRNA通道，tRNA通道和新生多肽出口通道的主要部分都包含Lmr特异性蛋白，这表明Lmr中募集mRNA，tRNA相互作用和新生多肽退出的机制必须与Lmr显着不同。推断其他生物体中核糖体的机制。我们的研究确定了某些仅是线粒体核糖体特征的结构特征，以及其他既是线粒体和叶绿体核糖体特征的特征（即细胞体核糖体）。

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    Manjuli R. Sharma; Timothy M. Booth; Larry Simpson; Dmitri A. Maslov; Rajendra K. Agrawal;

  • 作者单位

    Division of Translational Medicine, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201-0509;

    Division of Translational Medicine, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201-0509;

    Department of Microbiology, Immunology, and Molecular Genetics, David Geffen School of Medicine, University of California, Los Angeles, CA 90095;

    Department of Biology, University of California, Riverside, CA 92521;

    Division of Translational Medicine, Wadsworth Center, New York State Department of Health, Empire State Plaza, Albany, NY 12201-0509 Department of Biomedical Sciences, School of Public Health, State University of New York, Albany, NY 12201;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    cryo-EM; leishmania tarentolae; organellar ribosome;

    机译：低温电磁塔什坦白热病细胞核糖体;

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