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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Rho directs widespread termination of intragenic and stable RNA transcription
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Rho directs widespread termination of intragenic and stable RNA transcription

机译:Rho指导基因内和稳定RNA转录的广泛终止

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摘要

The transcription termination factor Rho is a global regulator of RNA polymerase (RNAP). Although individual Rho-dependent terminators have been studied extensively, less is known about the sites of RNAP regulation by Rho on a genome-wide scale. Using chromatin immu-noprecipitation and microarrays (ChIP-chip), we examined changes in the distribution of Escherichia coli RNAP in response to the Rho-specif ic inhibitor bicyclomycin (BCM). We found -200 Rho-terminated loci that were divided evenly into 2 classes: intergenic (at the ends of genes) and intragenic (within genes). The intergenic class contained noncoding RNAs such as small RNAs (sRNAs) and transfer RNAs (tRNAs), establishing a previously unappreciated role of Rho in termination of stable RNA synthesis. The intragenic class of terminators included a previously uncharacterized set of short antisense transcripts, as judged by a shift in the distribution of RNAP in BCM-treated cells that was opposite to the direction of the corresponding gene. These Rho-terminated antisense transcripts point to a role of non-coding transcription in E. coli gene regulation that may resemble the ubiquitous noncoding transcription recently found to play myriad roles in eukaryotic gene regulation.
机译:转录终止因子Rho是RNA聚合酶(RNAP)的全局调节因子。尽管已广泛研究了各个Rho依赖性终止子,但对Rho在全基因组范围内调控RNAP的位点知之甚少。使用染色质免疫沉淀和微阵列(ChIP芯片),我们检查了响应Rho特异性抑制剂双环霉素(BCM)的大肠杆菌RNAP的分布变化。我们发现-200个Rho末端位点被平均分为2类:基因间(基因末端)和基因内(基因内)。基因间的类包含非编码RNA,例如小RNA(sRNA)和转移RNA(tRNA),从而确立了Rho在稳定RNA合成终止中的前所未有的作用。内源性终止子类别包括先前未表征的一组短反义转录物,这可以通过RNA干扰在BCM处理的细胞中的分布变化来判断,该变化与相应基因的方向相反。这些Rho终止的反义转录物指出了非编码转录在大肠杆菌基因调控中的作用,该作用可能类似于最近发现的在真核基因调控中起多种作用的普遍存在的非编码转录。

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  • 作者单位

    Departments of Biochemistry, University of Wisconsin, Madison, WI 53706 Departments of Genetics, University of Wisconsin, Madison, WI 53706;

    Departments of Biochemistry, University of Wisconsin, Madison, WI 53706;

    Departments of Statistics, University of Wisconsin, Madison, WI 53706;

    Departments of Biochemistry, University of Wisconsin, Madison, WI 53706;

    Departments of Statistics, University of Wisconsin, Madison, WI 53706 Departments of Biostatistics and Medical Informatics, University of Wisconsin, Madison, WI 53706;

    Departments of Biochemistry, University of Wisconsin, Madison, WI 53706 Departments of Bacteriology, University of Wisconsin, Madison, WI 53706;

  • 收录信息 美国《科学引文索引》(SCI);美国《生物学医学文摘》(MEDLINE);美国《化学文摘》(CA);
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    chromatin immunoprecipitation; RNA polymerase;

    机译:染色质免疫沉淀RNA聚合酶;

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