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首页> 外文期刊>Molecular cell >MRNA Decapping Factors and the Exonuclease Xrn2 Function in Widespread Premature Termination of RNA Polymerase II Transcription
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MRNA Decapping Factors and the Exonuclease Xrn2 Function in Widespread Premature Termination of RNA Polymerase II Transcription

机译：在RNA聚合酶II转录广泛过早终止中的MRNA下降因素和核酸外切酶Xrn2功能。

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摘要

We report a function of human mRNA decapping factors in control of transcription by RNA polymerase II. Decapping proteins Edc3, Dcp1a, and Dcp2 and the termination factor TTF2 coimmunoprecipitate with Xrn2, the nuclear 5'-3' exonuclease " torpedo" that facilitates transcription termination at the 3' ends of genes. Dcp1a, Xrn2, and TTF2 localize near transcription start sites (TSSs) by ChIP-seq. At genes with 5' peaks of paused pol II, knockdown of decapping or termination factors Xrn2 and TTF2 shifted polymerase away from the TSS toward upstream and downstream distal positions. This redistribution of pol II is similar in magnitude to that caused by depletion of the elongation factor Spt5. We propose that coupled decapping of nascent transcripts and premature termination by the " torpedo" mechanism is a widespread mechanism that limits bidirectional pol II elongation. Regulated cotranscriptional decapping near promoter-proximal pause sites followed by premature termination could control productive pol II elongation.
机译：我们报告了由RNA聚合酶II的转录控制中人类mRNA决定因素的功能。脱盖蛋白Edc3，Dcp1a和Dcp2和终止因子TTF2与Xrn2共同免疫沉淀，而Xrn2是核5'-3'核酸外切酶“鱼雷”，可促进基因3'末端的转录终止。 Dcp1a，Xrn2和TTF2通过ChIP-seq定位在转录起始位点（TSS）附近。在具有暂停的pol II的5'峰的基因处，敲除或终止因子Xrn2和TTF2的敲低使聚合酶从TSS移向上游和下游远端位置。 pol II的这种重新分布在大小上与伸长因子Spt5耗尽所引起的相似。我们提出，通过“鱼雷”机制对新生转录本进行脱盖和过早终止是限制双向pol II延伸的广泛机制。在启动子附近的停顿位点附近进行调控的共转录去壳，然后过早终止可以控制生产性pol II的延伸。

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