...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Loss of a histone deacetylase dramatically alters the genomic distribution of Spo11p-catalyzed DNA breaks in Saccharomyces cerevisiae
【24h】

Loss of a histone deacetylase dramatically alters the genomic distribution of Spo11p-catalyzed DNA breaks in Saccharomyces cerevisiae

机译：组蛋白脱乙酰基酶的损失极大地改变了酿酒酵母中Spo11p催化的DNA断裂的基因组分布

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In eukaryotes, meiotic recombination events are distributed nonran-domly in the genome, with certain regions having high levels of recombination (hotspots) and others having low levels (coldspots). Species with similar DNA sequences (for example, chimpanzees and humans) can have strikingly different patterns of hotspots and coldspots. Below, by using a microarray analysis that allows us to measure the frequency of the meiosis-specific double-strand DNA breaks (DSBs) of all 6,000 yeast genes, we show that mutation of a single gene (SIR2), which encodes a histone deacetylase, significantly changes DSB frequencies of 12% of yeast genes, elevating DSBs of 5%, and reducing DSBs of 7%. Many of the genes with repressed recombination are located in large (50-100 kb) regions located near, but not at, the telomeres. Some of the genes with altered frequencies of DSBs (including the ribosomal RNA gene cluster) are known targets of Sir2p deacetylation in the wild-type strain.
机译：在真核生物中，减数分裂重组事件非随机地分布在基因组中，某些区域具有高水平的重组（热点），而其他区域具有低水平的重组（coldspots）。具有相似DNA序列的物种（例如黑猩猩和人类）可能具有截然不同的热点和冷点模式。下面，通过使用微阵列分析，我们可以测量所有6,000个酵母基因的减数分裂特异性双链DNA断裂（DSB）的频率，我们显示了编码组蛋白脱乙酰基酶的单个基因（SIR2）的突变显着改变了12％酵母基因的DSB频率，提高了5％的DSB，减少了7％的DSB。许多具有受阻重组的基因位于靠近端粒但不在端粒处的大区域（50-100 kb）。 DSB频率改变的某些基因（包括核糖体RNA基因簇）是野生型菌株中Sir2p脱乙酰基作用的已知靶标。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号