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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Insights into transcriptional regulation and σ competition from an equilibrium model of RNA polymerase binding to DNA
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Insights into transcriptional regulation and σ competition from an equilibrium model of RNA polymerase binding to DNA

机译：从RNA聚合酶与DNA结合的平衡模型中了解转录调控和σ竞争

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摘要

To explore scenarios that permit transcription regulation by activator recruitment of RNA polymerase and σ competition in vivo, we used an equilibrium model of RNA polymerase binding to DNA constrained by the values of total RNA polymerase (E) and 襼(70) per cell measured in this work. Our numbers of E and σ~(70) per cell, which are consistent with most of the primary data in the literature, suggest that in vivo (ⅰ) only a minor fraction of RNA polymerase ( < 20%) is involved in elongation and (ⅱ) σ~(70) is in excess of total E. Modeling the partitioning of RNA polymerase between promoters, nonspecific DNA binding sites, and the cytoplasm suggested that even weak promoters will be saturated with Eσ~(70) in vivo unless nonspecific DNA binding by Eσ~(70) is rather significant. In addition, the model predicted that σs compete for binding to E only when their total number exceeds the total amount of RNA polymerase (excluding that involved in elongation) and that weak promoters will be preferentially subjected to σ competition.
机译：为了探索允许通过激活RNA聚合酶的活化剂募集和体内σ竞争进行转录调控的场景，我们使用了一个RNA聚合酶与DNA结合的平衡模型，该模型受每个细胞中总RNA聚合酶（E）和襼（70）值的约束在这项工作中。我们每个细胞的E和σ〜（70）数与文献中的大多数主要数据一致，表明体内（ⅰ）只有一小部分RNA聚合酶（<20％）参与了伸长和（ⅱ）σ〜（70）超过总E.对启动子，非特异性DNA结合位点和细胞质之间的RNA聚合酶分配进行建模的研究表明，除非非特异性，否则即使弱的启动子在体内也会被Eσ〜（70）饱和。 Eσ〜（70）对DNA的结合非常重要。此外，该模型预测，仅当σs的总数超过RNA聚合酶的总量（不包括涉及延伸的σ）时，σs才会竞争与E的结合，并且弱启动子将优先受到σ竞争。

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