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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation
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Differential microarray analysis of Drosophila mushroom body transcripts using chemical ablation

机译：果蝇蘑菇体转录本的差异微阵列分析使用化学消融法

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摘要

Mushroom bodies (MBs) are the centers for olfactory associative learning and elementary cognitive functions in the Drosophila brain. As a way to systematically elucidate genes preferentially expressed in MBs, we have analyzed genome-wide alterations in transcript profiles associated with MB ablation by hydroxyurea. We selected 100 genes based on microarray data and examined their expression patterns in the brain by in situ hybridization. Seventy genes were found to be expressed in the posterodorsal cortex, which harbors the MB cell bodies. These genes encode proteins of diverse functions, including transcription, signaling, cell adhesion, channels, and transporters. Moreover, we have examined developmental functions of 40 of the microarray-identified genes by transgenic RNA interference; 8 genes were found to cause mild-to-strong MB defects when suppressed with a MB-Gal4 driver. These results provide important information not only on the repertoire of genes that control MB development but also on the repertoire of neural factors that may have important physiological functions in MB plasticity.
机译：蘑菇体（MBs）是果蝇大脑中嗅觉联想学习和基本认知功能的中心。作为系统地阐明在MBs中优先表达的基因的一种方法，我们分析了与通过羟基脲进行MB消融相关的转录谱中的全基因组变化。我们基于微阵列数据选择了100个基因，并通过原位杂交检查了它们在大脑中的表达模式。发现有70个基因在具有MB细胞体的后嗅皮层中表达。这些基因编码具有多种功能的蛋白质，包括转录，信号传导，细胞粘附，通道和转运蛋白。此外，我们已经通过转基因RNA干扰检查了40个微阵列识别基因的发育功能。发现有8个基因在被MB-Gal4驱动程序抑制后会引起轻度至强壮的MB缺陷。这些结果不仅提供了有关控制MB发育的基因谱系的重要信息，而且还提供了可能对MB可塑性具有重要生理功能的神经因子的谱系的重要信息。

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