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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Diversity of zebrafish peripheral oscillators revealed by luciferase reporting
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Diversity of zebrafish peripheral oscillators revealed by luciferase reporting

机译：萤光素酶报告揭示了斑马鱼外围振荡器的多样性

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摘要

In various multicellular organisms, circadian clocks are present not only in the central nervous system, but also in peripheral organs and tissues. In mammals peripheral oscillators are not directly responsive to light, but are entrained by the central oscillator in the suprachiasmatic nucleus. These individual oscillators are diverse in their free-running periods and phases. In contrast, cultured peripheral tissues and cell lines from zebrafish are not only rhythmic, but can also be directly entrained by light. Because of the convenience of studying rhythms in cultured cells, however, little has been known about properties of individual oscillators in intact zebrafish. Here, we show the remarkable diversity and consistency of oscillator properties in various peripheral organs and tissues from the period3-luciferase (per3-luc) transgenic zebrafish. Tissue-dependent differences were found in free-running period, phase, response to light, and temperature compensation. Furthermore, cycling amplitudes were reduced at lower temperatures in some, but not all, of the organs tested. Finally, we found that per3-luc rhythms can free run in both constant dark and constant light with remarkably similar amplitudes, phases, and periods, despite the fact that the mRNA of per2 and per1 has been shown not to oscillate in constant light.
机译：在各种多细胞生物中，昼夜节律钟不仅存在于中枢神经系统中，而且还存在于周围器官和组织中。在哺乳动物中，外围振荡器对光没有直接响应，但被chi上神经核中的中心振荡器所夹带。这些单独的振荡器的自由运行周期和相位各不相同。相反，来自斑马鱼的培养的外周组织和细胞系不仅有节奏，而且还可以被光直接夹带。由于方便地研究培养细胞的节律，因此，对于完整斑马鱼中单个振荡器的特性知之甚少。在这里，我们显示了来自perio3-luciferase（per3-luc）转基因斑马鱼的各种外围器官和组织中振荡器特性的显着多样性和一致性。在自由运行时间，相位，对光的响应和温度补偿方面发现了组织依赖性差异。此外，某些（但不是全部）受试器官在较低温度下循环振幅降低。最后，我们发现per3-luc节奏可以在恒定的黑暗和恒定的光线下自由运行，幅度，相位和周期都非常相似，尽管事实表明per2和per1的mRNA不会在恒定的光线下振荡。

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