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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >RNase P cleaves transient structures in some riboswitches
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RNase P cleaves transient structures in some riboswitches

机译：RNase P切割某些核糖开关中的瞬时结构

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摘要

RNase P from Escherichia coli cleaves the coenzyme B_(12) riboswitch from E. coli and a similar one from Bacillus subtilis. The cleavage sites do not occur in any recognizable structure, as judged from theoretical schemes that have been drawn for these 5′ UTRs. However, it is possible to draw a scheme that is a good representation of the E. coli cleavage site for RNase P and for the cleavage site in B. subtilis. These data indicate that transient structures are important in RNase P cleavage and in riboswitch function. Coenzyme B_(12) has a small inhibitory effect on E. coli RNase P cleavage of the E. coli riboswitch. Both E. coli RNase P and a partially purified RNase P from Aspergillus nidulans mycelia succeeded in cleaving a putative arginine riboswitch from A. nidulans. The cleavage site may be a representative of another model substrate for eukaryotic RNase P. This 5′ UTR controls splicing of the arginase mRNA in A. nidulans. Four other riboswitches in E. coli were not cleaved by RNase P under the conditions tested.
机译：来自大肠杆菌的RNA酶P切割来自大肠杆菌的辅酶B_（12）核糖开关和来自枯草芽孢杆菌的类似酶。从针对这些5'UTR绘制的理论方案判断，切割位点不会以任何可识别的结构出现。但是，可以绘制一个很好的表示RNase P和枯草芽孢杆菌中裂解位点的大肠杆菌裂解位点的方案。这些数据表明，瞬时结构在RNase P裂解和核糖开关功能中很重要。辅酶B_（12）对大肠杆菌核糖开关的大肠杆菌RNase P裂解具有很小的抑制作用。来自构巢曲霉菌丝体的大肠杆菌RNase P和部分纯化的RNase P均成功地从构巢曲霉中裂解了一个假定的精氨酸核糖开关。切割位点可以代表真核RNA酶P的另一种模型底物。该5'UTR控制构巢曲霉中精氨酸酶mRNA的剪接。在测试条件下，大肠杆菌中的其他四个核糖开关均未被RNase P切割。

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