...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >Functional similarities between phage λ Orf and Escherichia coli RecFOR in initiation of genetic exchange
【24h】

Functional similarities between phage λ Orf and Escherichia coli RecFOR in initiation of genetic exchange

机译：噬菌体λOrf与大肠杆菌RecFOR在基因交换启动中的功能相似性

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Genetic recombination in bacteriophage λ relies on DNA end processing by Exo to expose 3′-tailed strands for annealing and exchange by β protein. Phage λ encodes an additional recombi-nase, Orf, which participates in the early stages of recombination by supplying a function equivalent to the Escherichia coli RecFOR complex. These host enzymes assist loading of the RecA strand exchange protein onto ssDNA coated with ssDNA-binding protein. In this study, we purified the Orf protein, analyzed its biochemical properties, and determined its crystal structure at 2.5 A. The homodimeric Orf protein is arranged as a toroid with a shallow U-shaped cleft, lined with basic residues, running perpendicular to the central cavity. Orf binds DNA, favoring single-stranded over duplex and with no obvious preference for gapped, 3′-tailed, or 5′-tailed substrates. An interaction between Orf and ssDNA-binding protein was indicated by far Western analysis. The functional similarities between Orf and RecFOR are discussed in relation to the early steps of recombinational exchange and the interplay between phage and bacterial recombinases.
机译：噬菌体λ中的基因重组依赖于Exo对DNA的末端加工，以暴露3'尾链以进行退火并通过β蛋白进行交换。噬菌体λ编码另外的重组酶Orf，其通过提供与大肠杆菌RecFOR复合物等效的功能而参与重组的早期阶段。这些宿主酶有助于将RecA链交换蛋白加载到涂有ssDNA结合蛋白的ssDNA上。在这项研究中，我们纯化了Orf蛋白，分析了其生化特性，并确定了其在2.5 A时的晶体结构。同源二聚Orf蛋白排列成环形，带有浅U形裂口，内衬碱性残基，垂直于蛋白中央腔。 Orf结合DNA，倾向于单链而不是双链，并且对有缺口，3'尾或5'尾的底物没有明显的偏好。远西方分析表明Orf和ssDNA结合蛋白之间的相互作用。讨论了Orf和RecFOR之间的功能相似性，涉及重组交换的早期步骤以及噬菌体和细菌重组酶之间的相互作用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号