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首页> 外文期刊>Proceedings of the National Academy of Sciences of the United States of America >LacZ-expressing olfactory ensheathing cells do not associate with myelinated axons after implantation into the compressed spinal cord
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LacZ-expressing olfactory ensheathing cells do not associate with myelinated axons after implantation into the compressed spinal cord

机译：植入压缩脊髓后，表达LacZ的嗅鞘细胞不与髓鞘轴突结合

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摘要

Studies have shown that implanting olfactory ensheathing cells (OECs) may be a promising therapeutic strategy to promote functional recovery after spinal cord injury. Several fundamental questions remain, however, regarding their in vivo interactions in the damaged spinal cord. We have induced a clip compression injury at the T10 level of the spinal cord in adult rats. After a delay of 1 week, OECs isolated from embryonic day 18 rats were implanted into the cystic cavity that had formed at the site of injury. Before implantation, OECs were infected with a LacZ-expressing retrovirus. At 3 weeks after implantation, LacZ-expressing OECs survived the implantation procedure and remained localized to the cystic cavity. At the electron microscopic level, the cystic cavity had clusters of LacZ-expressing OECs and numerous Schwann cells lacking LacZ expression. Although labeled OECs made no direct contact with axons, unlabeled Schwann cells were associated with either a single myelinated axon or multiple unmyelinated axons. Positively labeled OEC processes often enveloped multiple Schwann cell-axon units. These observations suggest that the role of OECs as the primary mediators of the beneficial effects on axon growth, myelination, and functional recovery after spinal cord injury may require re-evaluation.
机译：研究表明，植入嗅鞘细胞（OEC）可能是促进脊髓损伤后功能恢复的有前途的治疗策略。然而，关于它们在受损脊髓中的体内相互作用，仍然存在一些基本问题。我们已经在成年大鼠的脊髓T10水平诱发了夹子压缩损伤。延迟1周后，将从胚胎第18天的大鼠中分离出的OEC植入受伤部位形成的囊性腔中。植入前，OEC被表达LacZ的逆转录病毒感染。植入后3周，表达LacZ的OEC在植入过程中幸存下来，并保留在囊腔中。在电子显微镜下，囊腔内有簇表达LacZ的OEC和许多缺少LacZ表达的雪旺氏细胞。尽管标记的OEC不与轴突直接接触，但未标记的雪旺氏细胞与单个髓鞘轴突或多个髓鞘轴突相关。带有正标记的OEC过程通常包裹着多个雪旺氏细胞轴突单元。这些观察结果表明，OEC作为脊髓损伤后轴突生长，髓鞘形成和功能恢复的有益作用的主要介质的作用可能需要重新评估。

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