...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Process Biochemistry >Improving the catalytic efficiency of Fibrinolytic enzyme from Serratia marcescens subsp. sakuensis by chemical modification
【24h】

Improving the catalytic efficiency of Fibrinolytic enzyme from Serratia marcescens subsp. sakuensis by chemical modification

机译：提高粘质沙雷氏菌亚种纤溶酶的催化效率。化学修饰的樱木

获取原文
获取原文并翻译 | 示例
   

获取外文期刊封面封底 >>

       
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Microbial fibrinolytic enzymes have gained increased attention due to their potential to prevent or cure cardiovascular diseases. Promising natural enzymes are often modified to improve/enhance the kinetic constants. Hence an attempt was made to chemically modify the fibrinolytic enzyme produced by marineSerratia marcescenssubsp.sakuensisusing amino acid specific modifiers. The aim was to enhance the kinetic constants and gather information on the vital amino acid residues involved in the catalysis. Modification of cysteine, histidine, tryptophan and serine residues resulted in drastic reduction in fibrinolytic activity indicating their presence in the active site. Modification of carboxylate residues resulted in a 19-fold increase in specific activity suggesting their presence in the catalytic site. Interestingly, ratio of fibrinolytic to fibrinogenolytic activity of the modified enzyme did not change significantly. There was a 507-fold reduction in Kmvalue after chemical modification and due to that, 219-fold enhancement of catalytic efficiency was evidenced. Circular dichroism spectrum analysis of the modified and native enzyme revealed changes in α- helix and ß-sheet conformation of the enzyme. Furthermore, the modified enzyme was more responsive to the presence of most of the metal ions tested.
机译：微生物纤维蛋白水解酶由于其预防或治疗心血管疾病的潜力而受到越来越多的关注。有前途的天然酶经常被修饰以改善/增强动力学常数。因此，尝试使用氨基酸特异性修饰剂化学修饰海洋粘质沙雷氏菌（Serraensis marcescenssubsp.sakuensis）产生的纤溶酶。目的是提高动力学常数并收集有关催化中重要氨基酸残基的信息。半胱氨酸，组氨酸，色氨酸和丝氨酸残基的修饰导致纤溶活性急剧降低，表明它们存在于活性位点。羧酸残基的修饰导致比活增加19倍，表明它们存在于催化位点。有趣的是，修饰酶的纤溶活性与纤溶酶活性之比没有明显变化。化学修饰后，Km值降低了507倍，因此，催化效率提高了219倍。修饰后的天然酶的圆二色光谱分析表明，该酶的α-螺旋和β-折叠构象发生了变化。此外，修饰的酶对大多数测试的金属离子的反应更灵敏。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号