• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Radiation and Environmental Biophysics >Tumor cell migration is not influenced by p21 in colon carcinoma cell lines after irradiation with X-ray or 12C heavy ions
【24h】

Tumor cell migration is not influenced by p21 in colon carcinoma cell lines after irradiation with X-ray or 12C heavy ions

机译：X射线或 12 C重离子辐照后，结肠癌细胞系中的肿瘤细胞迁移不受p21的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Metastasis and recurrences are major problems regarding an effective treatment of solid malignant tumors in clinical oncology. Since the impact of radiation on cell motility is not yet well understood, intrinsic and radiation-induced changes in cell migration have been discussed as possible mechanisms involved in the limitations of radiotherapy. This holds true for conventional radiation treatment and even more for the cellular and molecular effects of therapeutically relevant 12C heavy ions. The present study is therefore focused on the investigation of tumor cell migration in vitro after irradiation with X-rays and 12C heavy ions and on radiation-induced changes in the expression of proteins that are potentially relevant for motility. Two colon carcinoma cell lines, HCT116 and HCT116 p21−/−, were chosen for this study, which should be isogenic except for their p21-status. We can show here that cells lacking p21 react almost alike to radiation as wild type cells regarding survival and tumor cell migration 24 h after irradiation. Interestingly, differences in protein expression 24 h after irradiation of β1 integrin and protein kinase B isoforms Akt1 and Akt2 seem to exist. We conclude that tumor cell migration is unaffected by the p21-status in colorectal carcinoma cells and that the expression of the aforementioned proteins alone is not accountable for the differences observed.
机译：转移和复发是在临床肿瘤学中有效治疗实体恶性肿瘤的主要问题。由于尚未充分认识到辐射对细胞运动的影响，因此已经讨论了细胞迁移的固有和辐射诱导的变化，这是涉及放射疗法局限性的可能机制。对于常规放射治疗，甚至对于与治疗相关的 12 C重离子的细胞和分子效应，情况更是如此。因此，本研究的重点是在X射线和 12 C重离子辐照后体外肿瘤细胞迁移的研究，以及辐射诱导的与运动性相关的蛋白质表达变化。本研究选择了两种结肠癌细胞系HCT116和HCT116 p21-/-，除了它们的p21状态外，它们应该是同基因的。我们可以在这里证明缺乏p21的细胞对辐射的反应与野生型细胞几乎相同，这与辐射后24小时的存活和肿瘤细胞迁移有关。有趣的是，似乎在照射β 1 整联蛋白和蛋白激酶B同工型Akt1和Akt2后24小时蛋白表达存在差异。我们得出结论，肿瘤细胞迁移不受结直肠癌细胞中p21状态的影响，并且仅上述蛋白的表达不能解释观察到的差异。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号