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首页> 外文期刊>Sensors and Actuators >A two-photon fluorescent probe for endogenous superoxide anion radical detection and imaging in living cells and tissues
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A two-photon fluorescent probe for endogenous superoxide anion radical detection and imaging in living cells and tissues

机译：一种用于在活细胞和组织中内源性超氧阴离子自由基检测和成像的双光子荧光探针

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摘要

Superoxide anion radical (O_2~-), the "primary" reactive oxygen species (ROS) in living systems, is linked to a variety of physiological and pathological processes. Therefore, developing an effective strategy to monitor the fluctuation of O_2~- in biological systems is of great importance. This paper describes a new turn-on two-photon fluorescent probe for endogenous O_2~- detection and imaging, which was rationally designed and synthesized via a non-redox strategy. In the presence of O_2~-, the probe exhibited notable fluorescence enhancement (~235-fold) with a low detection limit down to 1 nM, indicating a high signal-to-background ratio and excellent sensitivity. In addition, short response time, good biocompatibility, low cytotoxicity, long-term stability against light illumination, specificity to O_2~- over general reductants, and pH stability were demonstrated, indicating that the requirements for cellular O_2~- determination are met. Furthermore, the probe was successfully applied in two-photon fluorescence imaging of endogenous O_2~- in living cells and tissues and showed high imaging resolution and a deep-tissue imaging depth of ~150 μm, illustrating the promising potential for practical applications in complex biosystems and providing a valuable theoretical basis and technical support for the study of physiological and pathological functions of O_2~-.
机译：超氧阴离子自由基（O_2〜-）是生命系统中的“主要”活性氧（ROS），与多种生理和病理过程有关。因此，制定一种有效的策略来监测生物系统中O_2〜-的波动具有重要意义。本文介绍了一种新型的用于内源性O_2〜-检测和成像的开启式双光子荧光探针，该探针是通过非氧化还原策略合理设计和合成的。在存在O_2〜-的情况下，该探针显示出显着的荧光增强（〜235倍），低至1 nM的低检测限，表明高的信噪比和出色的灵敏度。此外，还证明了响应时间短，生物相容性好，细胞毒性低，对光照的长期稳定性，对O_2〜-优于一般还原剂的特异性和pH稳定性，表明满足了对细胞O_2〜-测定的要求。此外，该探针已成功应用于活细胞和组织中内源性O_2〜-的双光子荧光成像，并显示出高成像分辨率和约150μm的深组织成像深度，说明了在复杂生物系统中的实际应用前景广阔为研究O_2〜-的生理和病理功能提供了有价值的理论依据和技术支持。

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  • 来源
    《Sensors and Actuators》 |2017年第10期|259-266|共8页
  • 作者

    Danqing Lu; Liyi Zhou; Ruowen Wang; Xiao-Bing Zhang; Lei He; Jing Zhang; Xiaoxiao Hu; Weihong Tan;

  • 作者单位

    Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China;

    Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China;

    Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China;

    Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China;

    Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China;

    Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China;

    Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China;

    Molecular Science and Biomedicine Laboratory, State Key Laboratory for Chemo/Bio Sensing and Chemometrics, College of Chemistry and Chemical Engineering, College of Biology, and Collaborative Research Center of Molecular Engineering for Theranostics, Hunan University, Changsha 410082, China;

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  • 关键词

    Endogenous O_2~- detection and imaging; Two-photon fluorescent probe; Turn-on response; Cell imaging; Tissue imaging;

    机译：内源性O_2〜-检测与成像;双光子荧光探针;开机响应;细胞成像;组织成像;

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