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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >A red emitting two-photon fluorescent probe for dynamic imaging of redox balance meditated by a superoxide anion and GSH in living cells and tissues
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A red emitting two-photon fluorescent probe for dynamic imaging of redox balance meditated by a superoxide anion and GSH in living cells and tissues

机译：红光双光子荧光探针，用于动态成像活细胞和组织中超氧阴离子和谷胱甘肽的氧化还原平衡

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摘要

Cellular self-regulation of reactive oxygen species (ROS) stress via antioxidant repair plays an important role in maintaining the redox balance. The redox balance between reducing and oxidizing species within cells is significant in the regulation of a signal pathway and is achieved by a series of elaborate mechanisms. In this work, we employed our previously reported D-pi-A-structured naphthalene-BODIPY TBET platform to design an efficient two-photon fluorescent probe for dynamic monitoring of superoxide anion oxidative stress and the GSH reducing repair process. The probe displayed high energy transfer efficiency (91.4%), large pseudo-Stokes shifts upon one-photon excitation, and red fluorescence emission (lambda(em) = 596 nm), which is highly desirable for bioimaging applications. The probe exhibits reversibility, rapid response, good photostability, high selectivity and sensitivity for the superoxide anion and GSH. More importantly, the probe was successfully applied for visualizing the redox changes in living cells and tissues.
机译：通过抗氧化剂修复，细胞对活性氧（ROS）应激的自我调节在维持氧化还原平衡中起着重要作用。细胞内还原性物质和氧化性物质之间的氧化还原平衡在信号通路的调节中很重要，并且是通过一系列复杂的机制实现的。在这项工作中，我们采用了我们先前报道的D-pi-A结构的萘-BODIPY TBET平台，设计了一种有效的双光子荧光探针，用于动态监测超氧阴离子氧化应激和GSH还原修复过程。该探针显示出高的能量转移效率（91.4％），单光子激发时的大伪斯托克斯位移和红色荧光发射（lambda（em）= 596 nm），这对于生物成像应用是非常需要的。该探针对超氧阴离子和GSH表现出可逆性，快速响应，良好的光稳定性，高选择性和敏感性。更重要的是，该探针已成功应用于可视化活细胞和组织中的氧化还原变化。

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